Yeast Media, Solutions and Stocks Protocols Protocol Links
Complex Yeast Media Protocols Complex yeast media protocols. Including: YPAD Medium or Also Called YPD plus Adenine; Synthetic Complete Drop Out (SC drop-out)Medium; Synthetic Complete drop-out Medium Mix (SC drop-out).
Long Term Storage of Yeast Stocks Protocol Yeast strains may be stored indefinitely at low temperatures (-80 degrees C). Two archiving methods are presented below. In Method A , the cells are grown on a plate, while in Method B the cells are grown in liquid culture.
Media and Culture of Yeast Protocol The yeast, Saccharyomyces cerevisiae, has become an important organism in molecular, biochemical, and genetic analysis. The organism has specific requirements for growth under a variety of conditions. The media, both liquid and solid, simple, define, and complex are describe in this unit. Also included are methods for handling, storing, and shipping stock of yeast.
Streaking Yeast Stocks Protocols Some yeast strains are unstable (e. g., small YAC-bearing strains) and need to be repurified by streaking on an agar plate and then verifying the genetic content of the isolated colony before proceeding. In cases where the strain is unstable, plan to streak the cells onto the selective medium to retain the desired stock, (however, most strains can be streaked onto the complete medium, YPD).