Xenopus Genetics Protocols

ENU Mutagenesis Protocol Protocol for ENU mutagenesis.

Isolating Xenopus Genomic DNA Protocol Protocol for isolating Xenopus genomic DNA.

Karyotyping Xenopus Embryos Protocol Protocol for karyotyping Xenopus embryos.

Xenopus cDNA Libraries Xenopus cDNA libraries. Includes: General cDNA clone providers.

Xenopus Chromatin immmunoprecipitation Protocol Protocol for Xenopus chromatin immunoprecipitation.

Xenopus DNA Fishing Protocol Protocol for Xenopus DNA fishing. Includes: DNA-agarose coupling reaction; PROTEIN BINDING REACTION; OLIGO ANNEALING.

Xenopus Fluorescent in situs and FCIS Protocol Protocols for performing wholemount fluorescent in situs. Instructions for two-color FISH and for a combination of fluorescent and colorimetric in situs we have called FCIS. Data can be imaged with a fluorescence stereoscope or a confocal.

Xenopus Gynogenesis Protocol Protocol for Xenopus gynogenesis.

Xenopus Oocyte Transfer Protocol Protocol for Xenopus oocyte transfer.

Xenopus RNA Isolation Protocol Protocol for Xenopus RNA isolation.

Xenopus Whole Mount In Situ Hybridization Protocols Protocols for Xenopus whole mount in situ hybridization. Includes: Protocols for Making Antisense RNA probe (dig labelled); Protocols for In Situ Hyb.