Cytostatic Factor (CSF) Extract Preparation for Xenopus Spindle Assembly Protocol Protocol for cytostatic factor extract preparation for spindle assembly. The quality of the eggs is essential for good CSF extracts. Always sacrifice quantity for quality when trying to make functional extracts. Discard any batches of eggs that have "puff balls" or activated eggs that constitute more than 10% of the eggs. Use laid eggs and collect eggs at about 16 to 17 hours after priming with Progesterone (found in Pregnant Mare Serum Gonadotropin (PMSG)).
Preparation of Xenopus Sperm Nuclei Protocol Protocol describes the preparation of nuclei from Xenopus sperm. These nuclei can be used for spindle assembly assays, microscopy, or DNA synthesis.
Xenopus Egg CSF Extract Preparation Protocol Protocol describes the preparation of Cytostatic Factor (CSF) from frog eggs. CSF can be used in in vitro spindle assembly assays. Includes: Frog injections; Preparation Before Starting the Extract Preparation; Extract Preparation.
Xenopus Sperm Nuclei Preparation Protocol Protocol for Xenopus sperm nuclei preparation. Sperm Nuclei can be made and stored as a stock for later use with Cytostatic Factor Extracts.
