Viral Protocols

Extraction of Geminiviral DNA from a Highly Mucilaginous Plant Protocol Protocol for the extraction of geminiviral DNA from a highly mucilaginous plant (Abelmoschus esculentus). Includes: Extraction; Alkali lysis; Cloning.

Gradient Purification of AT2 Inactivated HIV-1 Protocol Protocol for gradient purification of AT2 inactivated HIV-1.

HIV-1 p24 Antigen Capture ELISA Protocol Protocol for HIV-1 p24 antigen capture ELISA.

Purification of Bacteriophage lambda Arms: Centrifugation through Sucrose Density Gradients Protocol Protocol is used to prepare the arms of any bacteriophage {lambda} vector.

Purification of Bacteriophage lambda Particles by Isopycnic Centrifugation through CsCl Gradients Isopycnic centrifugation through CsCl gradients is used to prepare infectious bacteriophage {lambda} particles of the highest purity that are essentially free of contaminating bacterial nucleic acids.

Purification of Recombinant Adeno-Associated Virus (rAAV) and Parovirus Protocol Protocol for the purification of recombinant adeno-associated virus (rAAV) and parvovirus in pre-formed iodixanol gradients.

SDS-PAGE Sample Preparation of Adenovirus Infected Cells Protocol Protocol for SDS-PAGE sample preparation of adenovirus infected cells.

TEV Purification Protocol Protocol for TEV purification.

Titration of Retrovirus Protocol Protocol for the titration of retrovirus.

Viral Detection and Concentration in Food Methods and information related to viral detection and concentration in food. Includes: Nucleic acid amplification methods; Recent progress in polymerase chain reaction detection; Detection of human enteric viruses in non-shellfish food; Choice of primers for the detection of human caliciviruses in foods; Biosensors and microarray detection; Alternative confirmation methods.

Virion RNA Dot Blot Protocol Protocol describes the production of a dot blot membrane spotted with RNA isolated from virus particles.