A Protocol for AAV Vector Production and Purification Protocol describes typical methods that are used to propagate and purify AAV vectors for experiments both in vitro and in vivo. Includes: Principles of the Triple Plasmid Transfection System; Plasmids; Transfection and Extraction of Virus; Purification of the AAV vector.
Isolation of Infectious HIV-1 Protocol Protocol for the isolation of infectious HIV-1. Includes: HIV-1 capture strategy; Protocol for the isolation of HIV-1 virions; Magnetic labeling; Magnetic separation; Elution option A for virion lysate; Elution option B for intact virions.
Kit for Rapid Enrichment of SARS Virus During incubation period and the course of disease, SARS virus replicates and releases from cells. The level of virus varies greatly from sample to sample. At early stage of incubation period or during the late convalescent phase, the concentration of virus is very low in all kinds of samples. Virus is also detected at extremely low concentrations in plasma during...
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