T4 Polynucleotide Kinase Protocols. T4 Polynucleotide Kinase (T4 PNK) catalyzes
the transfer of the g-phosphate of ATP to the
5'-terminus of single- and double-stranded DNA
or RNA molecules that have a 5'-OH. Used for End-labeling of DNA and Phosphorylation of oligodeoxynucleotides with T4 PNK. Methods for phosphorylation of vector and plasmids with T4 PNK.