Long PCR Protocol Protocol can be used to amplify DNA up to 25 kb in length. To reduce the chance of contamination with exogenous DNAs, prepare and use a special set of reagents and solutions for PCR only. Bake all glassware for 6 hours at 150°C and autoclave all plasticware.
PCR Amplification of DNA PCR Amplification of DNA. Very Basic Protocol. Julie B. Wolf, University of Maryland.
PCR and Multiplex Guide PCR and Multiplex Guide. Amazing Guide to primer amount, MgCl2 amount, annealing temperature, and more. With picture examples. Octavian Henegariu.
Polymerase Chain Reaction (PCR) Lecture Polymerase Chain Reaction (PCR) Lecture - Properties and Choices of Polymerase, Basic Protocol for PCR. Dr. Michael Blaber
The Basic Polymerase Chain Reaction Protocol Protocol describes how to amplify a segment of double-stranded DNA in a chain reaction catalyzed by a thermostable DNA polymerase. It is the foundation for all subsequent variations of the polymerase chain reaction.