Spectral Karyotyping SKY Protocols

Amplification of Flow Sort DNA using DOP-PCR (SKY) Protocol Protocol for the amplification of flow sort DNA using DOP-PCR (SKY).

Applications of SKY in Cancer Cytogenetics SKY has been applied to various tumor groups including hematological malignancies, sarcomas, carcinomas and brain tumors, with the intent of identifying specific chromosomal abnormalities that may provide insight to the genes involved in the disease process as well as identifying recurrent cytogenetic markers for clinical diagnosis and prognostic assessment.

Detection of Chromosomal Imbalances using DOP-PCR and Comparative Genomic Hybridization (CGH) Protocol for the Detection of chromosomal imbalances using DOP-PCR and comparative genomic hybridization (CGH). Includes: Preparation and labeling of DOP-PCR products; Comparative genomic hybridization (CGH).

Detection SKY Protocol Protocol for detection SKY.

DOP-PCR Labeling SKY Protocol Protocol for DOP-PCR Labeling (SKY).

DOP-PCR Secondary SKY Protocol Protocol for DOP-PCR Secondary (SKY).

Hybridization SKY Protocol Protocol for hybridization (SKY).

Precipitation of SKY Kits and QC Protocol Protocol for precipitation of SKY Kits and QC.

Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol Protocol for pretreatment of chromosome slides for FISH/CGH/SKY.

SKY/FISH of Previously G-Banded Slides Protocol Protocol for SKY/FISH of previously G-Banded slides. Includes: G-Band Slide Pretreatment; G-Band Slide Denaturation and Hybridization.

Spectral Karyotyping Mouse Protocol Protocol for spectral karyotyping mouse.