FP-TDI Method for SNP Detection FP-TDI Method for SNP Detection. The FP-TDI protocol was originally reported by Drs. Chen, Levine, and Kwok at Washington University in 19991,2. FP-TDI stands for template directed dye terminator incorporation assay with detection by fluorescence polarization. It is a single base primer extension assay couple with homogeneous FP detection. Perkin Elmer
High-resolution SNP mapping by denaturing HPLC High-resolution SNP mapping by denaturing HPLC. A SNP mapping procedure that relies on resolving polymorphisms by denaturing HPLC without the necessity of determining the nature of the SNPs. They demonstrate the use of denaturing high-performance liquid chromatography to identify mutations in the candidate genes and to fine-map chromosomal breakpoints.
SNP detection with mutagenic primers SNP detection with mutagenic primers. Input sequence will be searched to find changes in one nucleotide near the location of the SNP, so that mutagenic primers may be easily designed. Bikandi, J., San MillÃ¡n, R., Rementeria, A., and Garaizar, J. In silico analysis of complete bacterial genomes: PCR, AFLP-PCR, and endonuclease restriction. Bioinformatics 2004 Mar 22;20(5):798-9.
SNP Restriction Digestion Comparison Compare restriction patterns of two sequences
for SNP/Mutation detection. Online program that compares restriction enzyme digest of DNA and finds the differences between two sequences.