RNA interference protocol RNA interference protocol. Preparation of Template DNA, Preparation of the dsRNA, RNAi in Drosophila Schneider cells. Matthias Wilm.
RNA Interference Screening (RNAi) in Cultured Cells The protocol listed is Drosophila cells in 6 well plates and our pre-aliquoted 384 well plates. RNAi experiments may be done in other size plates, just scale up or down. Drosophila RNAi Screening Center
The siRNA user guide Selection of siRNA duplexes from the target mRNA sequence, Preparation of siRNA duplexes, Useful siRNA reagent combinations, Transfection of siRNA duplexes, Sequences of siRNA duplexes used in our studies. Tuschl Lab.