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Signalling Signal Transduction Protocols

Signalling Protocols, and Signal Transduction methods and Protocols. Chemokines, Cytokine Analysis, and phosphorylation measurements. 

Protocols » Signalling Signal Transduction Protocols

Signalling Signal Transduction Protocols Categories

Calcium Measurement Protocols (10)

Chemokine Protocols (4)

Cytokine Protocols (6)

MAPK Protocols (11)

Protein Kinase Protocols (5)


Signalling Signal Transduction Protocols Protocol Links



Submitted Signalling Signal Transduction Protocols Articles

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

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