Protocols and information related to RNAi technology. Includes: RNAi in Avian Embryos; RNAi in C. elegans; RNAi in Drosophila; RNAi in Mammalian Cells; RNAi in Mouse Embryos & Oocytes RNAi in Other Cell Types.
Detect and Quantitate MicroRNA in Laser Capture Microdissection Samples Information on how detect and quantitate MicroRNA in laser capture microdissection samples. Includes: Obtain Laser Capture Microdissected Samples; Isolate miRNA from LCM Samples; Quantitate miRNA by qRT-PCR; Detection of miRNA in Microdissected Tissue from Mouse Brain by qRT-PCR; Differential Expression of MicroRNA in Whole Brain Tissue Compared to a More Homogeneous Population of Cells.
Protocol for siRNA-Primed RNA Synthesis Protocol Short interfering RNAs (siRNAs) can be used to prime RNA synthesis by the RNA-dependent RNA polymerase (RdRP). SiRNAs can be used by RdRP as primers for specific cellular mRNAs, forming dsRNA products capable of inducing transitive RNAi.
The siRNA User Guide The siRNA user guide. Includes: Selection of siRNA duplexes from the target mRNA sequence; Preparation of siRNA duplexes; Useful siRNA reagent combinations; Transfection of siRNA duplexes; Sequences of siRNA duplexes used in our studies.