RNAi Mouse Embryos and Oocytes Protocols Protocol Links
Collection of Early Mouse Embryos for RNAi Protocol Protocol describes a method to collect early embryos from 6-week-old mice. Subsequently, the isolated embryos can be injected with double-stranded RNA to induce knockdown of a gene of interest.
Collection of Mouse Oocytes for RNAi Protocol Protocol describes a method to collect oocytes from 6-week-old mice. Subsequently, the isolated oocytes can be injected with double-stranded RNA to induce knockdown of a gene of interest.
Microinjection of dsRNA into Mouse Oocytes and Early Embryos Protocol Protocol describes how to introduce a double-stranded RNA (dsRNA) of choice into mouse oocytes or fertilized one-cell embryos by microinjection. For collection of mouse oocytes and early embryos, see Collection of Mouse Oocytes for RNAi and Collection of Early Mouse Embryos for RNAi.