Cosuppression in C. elegans Protocol Cosuppression is a process in Caenorhabditis elegans that closely resembles RNAi. In contrast to RNAi, however, the cosuppression effect in C. elegans does not spread throughout the animal. Cosuppression in C. elegans can be triggered by highly repetitive transgenes that contain gene constructs.
Detection of siRNA in C. elegans Using Rnase Protection Protocol Protocol uses Rnase protection to detect short interfering RNAs (siRNAs) in RNA preparations from Caenorhabditis elegans. SiRNAs can also be detected by northern blot. However, the Rnase protection assay seems to be more sensitive.
Introduction of Double-Stranded RNA in C. elegans by Feeding Protocol Protocol describes an easily scalable way of introducing double-stranded RNA (dsRNA) in Caenorhabditis elegans: feeding the nematode with bacteria that express dsRNA. When using an Rnase-III-negative Escherichia coli strain (HT115), the efficiency of this method is comparable to the alternative.
Introduction of Double-Stranded RNA in C. elegans by Injection Protocol Double-stranded RNA (dsRNA) can be efficiently introduced into Caenorhabditis elegans by microinjection into the gonad, the gut, or the body fluid. The RNAi effect will spread within the nematode, exerting an effect beyond the site of injection.
Introduction of Double-Stranded RNA in C. elegans by Soaking Protocol Double-stranded RNA (dsRNA) can be introduced into Caenorhabditis elegans by soaking the animals in a solution of dsRNA. Alternative methods are dsRNA injection and feeding the animals with bacteria that produce dsRNA.
RNAi Screens in C. elegans Protocol Protocol for RNAi screens in C. elegans in a 96-well liquid format and their application to the systematic identification of genetic interactions. The procedure allows thousands of RNAi feeding experiments to be performed per investigator per day.
