Protocols and information related to RNA isolation and purificiation. Including: RNA Isolation from Microorganisms; RNA Isolation from Tissues or Cultured Cells.
Extraction and Purification of Total RNA using TRIzol OR TRI Reagent Protocol Protocol for extraction and purification of total RNA using TRIzol OR TRI reagent. Includes: Homogenization for Cell Suspensions; Phase Separation; RNA Precipitation; RNA Wash; Redissolving the RNA; Determination of RNA Concentration and Purity; Preparation of Rnase-free water.
RNA Affinity Isolations with PUMILIO from Drosophila melanogaster Protocol Protocol for RNA affinity isolations with PUMILIO from Drosophila melanogaster.
RNA Isolation Protocol Protocol for RNA isolation.
Total RNA isolation (Guanidinium Thiocyanate-Phenol-Chloroform Extraction) Protocol Protocol for total RNA isolation. This method is fast and works well for preparation of total RNA. We typically use total RNA for quantitative S1 nuclease analysis of endogenous (or in vitro) gene expression, and this method works well for this purpose.
DNA Protocols - DNA Cloning
Cell Biology Protocols - Cell Culture Protocols
PCR Protocols Protein Protocols
Western Blot Protocols
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