Protein Ubiquitination Protocols. Ubiquitin (Ub) is a conserved 76 amino acid protein found in all eukaryotic cells. Ub is covalently ligated to a variety of intracellular proteins through an isopeptide linkage. Ubiquitination has been implicated in the regulation of diverse cellular processes, such as selective protein breakdown, cell cycle regulation, and stress response. Protocols for ubiquitination include Deubiquitinating Enzyme assays, ubiquitin assays.
A Method for Assaying Deubiquitinating Enzymes A Method for Assaying Deubiquitinating Enzymes. Lee et al., Biol Proced Online. May 1998. A general method for the assay of deubiquitinating enzymes was described in detail using 125I-labeled ubiquitin-fused αNH-MHISPPEPESEEEEEHYC (referred to as Ub-PESTc) as a substrate.
A specific endpoint assay for ubiquitin. A specific endpoint assay for ubiquitin. Rose et al., Proc Natl Acad Sci U S A. 1987. Simple endpoint assays for free ubiquitin (Ub) and for the Ub-activating enzyme are described. .
Ultimate His-UB Assay for Mammalian Cells Ultimate His-UB Assay for Mammalian Cells. Tansey Lab Protocols. William P. Tansey. PREPARATION OF NI-NTA-AGAROSE, HARVESTING THE TRANSFECTION., ELUTION OF HIS-TAGGED PROTEINS.
