Manual Microdissection There are several manual methods that can be used to perform tissue microdissection. Techniques using hand-held tools as well as mechanical micromanipulator-based approaches have been described. However, speed and precision are the most important parameters and any method that achieves these is adequate. Investigators should also expect to invest time initially by practicing on 10 to 20 cases to begin to feel comfortable with the technique.
Microdissection Overview Overview of microdissection. Includes: The Goals of Microdissection; The Development of Microdissection.
Protein Staining Protocols Protocols for protein staining. Includes: Coomassie Blue Staining; Silver Nitrate Staining; Zinc Imidazole Negative Staining.
Slide Preparation for Manual Microdissection Protocol Slide Preparation for Manual Microdissection for Subsequent DNA, RNA, and Protein Analysis. Manual microdissection and subsequent molecular analysis can be carried out on slides stained using standard hematoxylin and eosin methods. However, if cell types that are (or are not) expressing a specific protein are required for a study, then more advanced slide preparation methods such as Immuno-LCM may be utilized.
Static Culture of Postimplantation Embryos for Imaging Protocol This protocol describes a method for static culture of early postimplantation mouse embryos on a microscope stage. Embryos between 6.5 and 9.5 days post coitum (dpc) can be cultured and imaged for 24 hours, with very little growth retardation.
Targeted Differential Display Protocol The protocol is one example of differential gene expression analysis of cells obtained from microdissected tissue. Includes: Microdissection and RNA Isolation; Reverse Transcription; PCR; P.A.G.E.; Sequencing of Differentially Expressed Bands.
