Nucleic Acid Labeling Protocols

Estimating the Yield of DIG-Labeled Nucleic Acids Protocol Protocol for estimating the yield of DIG-labeled nucleic acids.

Fill-in Labeling of DNA Fragments Protocol Protocol for fill-in labeling of DNA fragments.

Genomic DNA Labeling Protocol Protocol for genomic DNA labeling.

Genomic DNA Labeling Protocol II Protocol for genomic DNA labeling.

Hybridization of Oligonucleotide Probes in Aqueous Solutions Protocol Hybridization is carried out in conventional aqueous solvents at a temperature well below the predicted melting temperature.  Nonspecific hybrids are then removed by washing at high stringency in buffers containing quaternary salts.  Tetramethylammonium chloride (TMACl) is used with probes that are 14-50 nucleotides in length, whereas tetraethylammonium chloride (TEACl) is used with longer oligonucleotides.

Nick-Translation Labeling of ds DNA with Nick Translation Mixes for In Situ Probes Protocol Protocol for nick-translation labeling of ds DNA with nick translation mixes for in situ probes. Includes: Labeling reaction with DIG-dUTP or Biotin-dUTP; Labeling reaction with Fluorescein-dUTP, or Tetramethylrhodamine-dUTP; Purification of labeled probe.

Purification of Labeled Probes Using the High Pure PCR Product Purification Kit Protocol Protocol for purification of labeled probes using the High Pure PCR Product Purification Kit.