Nuclear Extraction Protocols. Extraction methods and procedures for nuclear proteins from the nucleus.
Large scale nuclear extract preparation Homogenize cells or tissue, Pellet nuclei, Lyse nuclei, Reprecipitate nuclear proteins, dialyze nuclear extract. Hattori M et al., DNA Cell Biol. 1990. Homogenization buffer and dialysis buffer recipes.
Nuclear and Splicing Extract Preparation S100 extract cytosolic extraction preparation. Buffer A (Hypotonic Buffer), Low and High salt buffer recipes. Brent Graveley
Preparation of Nuclear Extracts for Gel Shifts and Westerns Blots Nuclear Extract protocol is optimized for about 10^7 cells, can use for gel shift assays and western blotting. Mirmira Lab, Univ. Virginia
Yeast Nuclear Extract (Small Scale) Nuclear Extract preparation protocols, Buffers and solutions for nuclear extracts. Hahn Lab
DNA Protocols - DNA Cloning
Cell Biology Protocols - Cell Culture Protocols
PCR Protocols Protein Protocols
Western Blot Protocols
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