Nuclear Extraction Protocols

Large scale nuclear extract preparation Homogenize cells or tissue, Pellet nuclei, Lyse nuclei, Reprecipitate nuclear proteins, dialyze nuclear extract. Hattori M et al., DNA Cell Biol. 1990. Homogenization buffer and dialysis buffer recipes.

Nuclear and Splicing Extract Preparation S100 extract cytosolic extraction preparation. Buffer A (Hypotonic Buffer), Low and High salt buffer recipes. Brent Graveley

Preparation of Nuclear Extracts for Gel Shifts and Westerns Blots Nuclear Extract protocol is optimized for about 10^7 cells, can use for gel shift assays and western blotting. Mirmira Lab, Univ. Virginia

Yeast Nuclear Extract (Small Scale) Nuclear Extract preparation protocols, Buffers and solutions for nuclear extracts. Hahn Lab