Neurospora Protocols

Cryofixation and Freeze Substitution for Ultrastructural Studies of Neurospora crassa hyphae Protocol for cryofixation and freeze substitution for ultrastructural studies of Neurospora crassa hyphae.

Generation of Transformable Spheroplasts Protocol Protocol for generation of transformable spheroplasts from mycelia, macroconidia, microconidia and germinating ascospores of Neurospora crassa.

Hints and Precautions for the Care, Feeding and Breeding of Neurospora Hints and precautions for the care, feeding and breeding of Neurospora. Includes: Crossing; Tips on handling ascospoes General Laboratory Practices; Stockkeeping; Mutagenesis and enrichment; Tips for encouraging colonial growth; Solutions and media; Handling Heterokaryons.

How and Where to Obtain Information on Neurospora Information and tips on how and where to obtain information on Neurospora.

How to Activate Ascospores and Optimize the Recovery of Progeny Information and tips on how to activate ascospores and optimize the recovery of progeny.

How to Amplify DNA from Macroconidia Information and tips on how to amplify DNA from macroconidia.

How to Assay Light Responses in Neurospora Information and tips on how to assay light responses in Neurospora.

How to Avoid Contamination by Airborne Conidia Information and tips on How to avoid contamination by airborne conidia.

How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab Information and tips on how to avoid pitfalls and keep out of trouble in the Neurospora lab. Includes: Culture, incubation, storage, and disposal; Stock preservation; Spore and colony counts; Making crosses and recovering progeny; Use of heterokaryons for allelism tests; Use of unordered asci to estimate the relation of translocation breakpoints to centromeres.

How to Certify that Neurospora is Nonhazardous Information and tips on how to certify that Neurospora is nonhazardous.

How to Choose and Prepare Media Information and tips on how to choose and prepare media.

How to Choose Wild Type Laboratory Strains of N. crassa for Reference and for Special Uses Information and tips on how to choose wild type laboratory strains of N. crassa for reference and for special uses.

How to Color-Code Media Information on how to color-code media.

How to Convert Wild-Type Spreading Growth to Colonial Information and tips on how to convert wild-type spreading growth to colonial.

How to Create Gene Knockouts in Neurospora Information and tips on how to create gene knockouts in Neurospora.

How to Deal with Mite Infestations and Minimize their Occurrence Information and tips on how to deal with mite infestations and minimize their occurrence.

How to Demonstrate Barrage in Neurospora Information and tips on how to demonstrate barrage in Neurospora.

How to Deposit Materials in the Fungal Genetics Stock Center (FGSC) Information and tips on how to deposit materials in the Fungal Genetics Stock Center (FGSC).

How to Determine Gene Order Using 3-Point Crosses Information and tips on how to determine gene order using 3-point crosses.

How to Determine Mating Type Information and tips on how to determine mating type.

How to Determine Recessive-Lethal Mutation Rates Information and tips on how to determine recessive-lethal mutation rates.

How to Determine the Species of a Wild-Collected Isolate Information and tips on how to determine the species of a wild-collected isolate.

How to Determine Whether a Gene is Essential for Survival Information and tips on how to determine whether a gene is essential for survival.

How to Determine Whether a Strain will Undergo Senescence Information and tips on how to determine whether a strain will undergo senescence.

How to Distinguish a Spore Killer From Alternative Causes of Ascospore Abortion Information and tips on how to distinguish a Spore killer from alternative causes of ascospore abortion.