Mouse Protocols

BDA Mouse Lineage Tracing on Sections Protocol Protocol describes the strongest way for lineage tracing on sections by using biotin dextran amine.

Calcium Dependent by Pentraxins to Ligands, and Effect of C-reactive Protein on Leptin Action Protocol for calcium dependent by pentraxins to ligands immobilized on agarose, and effect of C-reactive protein on leptin action in mice in vivo. Includes: Testing for binding of CRP to immobilized leptin; Testing for binding of radiolabelled leptin by immobilized CRP, SAP and leptin receptor; Testing for effect of human CRP on human leptin action in mice.

Collection of Mouse Peritoneal Cells Protocol Protocol for the collection of mouse peritoneal cells.

Dissociation of Mouse Spleen and Hemopoietic Tissue Protocol Protocol for the dissociation of mouse spleen and hemopoietic tissue.

Effects of Cyclopamine on Mouse Vibrissa Follicle Explants Protocol to test the effects of cyclopamine on mouse vibrissa follicle explants.

Extraction of the SecinH3 from Mouse Liver Protocol Mice fed with the cytohesin inhibitor SecinH3 for two days develop hepatic insulin resistance that can be identified by reduced liver glycogen levels, increased serum insulin and ketone body levels and decreased serum non-esterified fatty acid. To confirm the presence and identity of SecinH3 in mouse liver, we extracted the compound from liver homogenates with chloroform and identified it by LC/MS.

Immunohistology of Mouse Spleens Protocol Protocol for the immunohistology of mouse spleens.

Isolation of Mouse Intrahepatic Lymphocytes Protocol Provides two protocols that may be used to isolate IHL. One can be used to isolate IHL from multiple livers in parallel, whereas the more elaborate alternate protocol yields more cells per liver but is more appropriately used to recover the IHL from a single liver.

Isolation of Murine Macrophages Protocol Protocol that investigates macrophage activation using a more homogeneous population of cells, macrophages derived from immature progenitor cells in bone marrow can be studied. A second protocol describes the isolation of bone marrow-derived progenitor cells and propagation of these immature macrophages by CSFs or IL-3.

Natural Pathogens of Laboratory Mice and Rats For all the transgenic or other mouse researchers out there who have had to explain why colony infections are not just bad for the welfare of the animals concerned, but may invalidate the ongoing studies as well.