Protocols and information related to mice. Including: Mouse Surgical Procedures; Mouse Reproductive System Surgery; Mouse Organ Removal Surgery; Assembling Chimeras; Mouse Embryology; Mouse Genetics; Mouse Cell Culture.
Extraction of the SecinH3 from Mouse Liver Protocol Mice fed with the cytohesin inhibitor SecinH3 for two days develop hepatic insulin resistance that can be identified by reduced liver glycogen levels, increased serum insulin and ketone body levels and decreased serum non-esterified fatty acid. To confirm the presence and identity of SecinH3 in mouse liver, we extracted the compound from liver homogenates with chloroform and identified it by LC/MS.
Isolation of Mouse Intrahepatic Lymphocytes Protocol Provides two protocols that may be used to isolate IHL. One can be used to isolate IHL from multiple livers in parallel, whereas the more elaborate alternate protocol yields more cells per liver but is more appropriately used to recover the IHL from a single liver.
Isolation of Murine Macrophages Protocol Protocol that investigates macrophage activation using a more homogeneous population of cells, macrophages derived from immature progenitor cells in bone marrow can be studied. A second protocol describes the isolation of bone marrow-derived progenitor cells and propagation of these immature macrophages by CSFs or IL-3.
Natural Pathogens of Laboratory Mice and Rats For all the transgenic or other mouse researchers out there who have had to explain why colony infections are not just bad for the welfare of the animals concerned, but may invalidate the ongoing studies as well.