Electroporating DNA into Embryonic Stem (ES) Cells and Selection Methods Protocol Protocol describes a method for electroporating DNA into ES cells, as well as selection methods. Pilot studies should be performed to optimize the conditions for each DNA construct. The selection method described here is one of the most complex. It involves targeting constructs in which the bacterial neomycin-resistance gene disrupts the coding sequence of the mouse gene.
PCR of Blood, Hair or Small Tissue Samples of Mouse Protocol Protocol for the PCR of blood, hair or small tissue samples of mouse. Includes: Preparation of blood or a tissue sample obtained by ear punch for PCR; Preparation of hair shafts for PCR.
Preparation of Genomic DNA from Mouse Tails and Other Small Samples Protocol Method is used chiefly to genotype transgenic and knockout mice. Each 6-10-mm snippet of mouse tail yields 50-100 µg of DNA that can be used in dot or slot blotting to detect a transgene of interest, in Southern hybridization to detect DNA fragments that are <20 kb in size, and as a template in PCRs.