Mouse Genetics Protocols

Electroporating DNA into Embryonic Stem (ES) Cells and Selection Methods Protocol Protocol describes a method for electroporating DNA into ES cells, as well as selection methods. Pilot studies should be performed to optimize the conditions for each DNA construct. The selection method described here is one of the most complex. It involves targeting constructs in which the bacterial neomycin-resistance gene disrupts the coding sequence of the mouse gene.

Mouse Tail DNA Extraction Protocol Protocol for mouse tail DNA extraction.

PCR of Blood, Hair or Small Tissue Samples of Mouse Protocol Protocol for the PCR of blood, hair or small tissue samples of mouse. Includes: Preparation of blood or a tissue sample obtained by ear punch for PCR; Preparation of hair shafts for PCR.

Preparation of Genomic DNA from Mouse Tails and Other Small Samples Protocol Method is used chiefly to genotype transgenic and knockout mice. Each 6-10-mm snippet of mouse tail yields 50-100 µg of DNA that can be used in dot or slot blotting to detect a transgene of interest, in Southern hybridization to detect DNA fragments that are <20 kb in size, and as a template in PCRs.

Simple, Reliable Steps for DNA Fragment Isolation and Purification Protocol Protocol describes a simple procedure for DNA fragment isolation and purification. The resulting DNA fragment can then. be used for microinjection to produce transgenic mice

Tail DNA Preps for Genomic Southerns Protocol Protocol for tail DNA preps for genomic southerns.

Triple Color FISH Technique for Mouse Chromosome Identification Protocol Protocol for triple color FISH technique for mouse chromosome identification.

Vasectomy of Mouse Protocol Protocol for the vasectomy of mouse. Includes: The Anesthetic; Test Breeding.