Mouse Embryonic Fibroblasts Protocols

ES / MEF Cell Culture and Electroporation of Targeting Construct Protocol Protocol for ES/MEF cell culture and electroporation of targeting construct.

Propagation of Pluripotent Mouse Embryonic Stem (ES) Cells Protocol This protocol describes the culture of embryonic stem (ES) cells using mitotically inactivating primary mouse embryonic fibroblast (MEF) cells as a feeder layer (preparation described in Preparation of Mouse Embryonic Fibroblast (MEF) Feeder Plates). The ES culture medium is supplemented with recombinant leukemia inhibitory factor (LIF) to help maintain the cells as pluripotent stem cells. This protocol has been optimized for the ES-D3 cell line.

Splitting Human ES cells on MEFs Protocol Protocol for splitting human ES cells on MEFs.