Collecting Blastocysts Protocol Protocol describes a method for collecting blastocysts from pregnant female mice at 3.5 to 4.5 days post coitum (dpc). The blastocysts can then be injected with embryonic stem cells to make chimeras.
Isolating Mice Extraembryonic Membranes Protocol Isolation of extraembryonic tissues allows one to study normal mouse development as well as the molecular basis of defects which cause fetal death. This protocol describes a method for isolating extraembryonic membranes from pregnant mice.
Making Injection Pipettes Protocol Protocol describes the determination of useful settings with the Sutter Puller P-97 to make injection pipettes for microinjection.
Planning an Effective Mouse Colony Considerations in planning an effective mouse colony for transgenic production. Includes: Mouse strain; Colony size; Efficiency of superovulation; Parental Suitability; Pseudo-pregnancy.
Preparing Siliconized Pipettes Protocol Protocol describes a method for preparing siliconized pipettes. Such pipettes minimize the loss of embryos or embryonic tissues during transfer.
Sectioning Mouse Embryos Protocol Protocol provides methods and tips for sectioning mouse embryos and transferring the sections to a microscope slide.
Transfer of Injected Blastocysts to Pseudo Pregnant Mice Protocol Blastocyst transfer is usually performed 24 hours after aggregation, when the morulae have become expanded blastocysts, and on the same day as injection. A little time is given between injection and transfer to allow the blastocysts to re-expand. Includes: The Mouse Recipient; The Transfer.
Whole Mount In Situ Hybridization of Mouse Protocol Protocol for whole mount in situ hybridization of mouse. Incluses: Preparation of the probe; General Comments; Preparation of embryos; In Situ hybridization.