Mouse Embryology Protocols

Blastocyst Embryo Transfer into Pseudopregnant Mouse Protocol Protocol for blastocyst embryo transfer into pseudopregnant recipient female mouse.

Breeding Mice Protocol Information and protocol for breeding mice.

Collecting Blastocysts Protocol Protocol describes a method for collecting blastocysts from pregnant female mice at 3.5 to 4.5 days post coitum (dpc). The blastocysts can then be injected with embryonic stem cells to make chimeras.

Collecting Zygotes and Removing Cumulus Cells With Hyaluronidase Protocol Protocol describes a method for collecting zygotes from pregnant female mice. The zygotes can then be used for microinjection.

Detection of Estrus in Mice Information on the detection of estrus in mice.

Disaggregating Cleavage-Stage Embryos and the Inner Cell Mass of Blastocysts into Individual Cells Protocol describes a method for disaggregating embryos and the ICM of blastocysts into individual cells.

Fixation of Mouse Embryos and Tissues Protocol Protocol provides methods for fixation of mouse embryos and tissues with paraformaldehyde, Bouin’s fixative, or methanol/DMSO solution.

Isolating Mice Extraembryonic Membranes Protocol Isolation of extraembryonic tissues allows one to study normal mouse development as well as the molecular basis of defects which cause fetal death. This protocol describes a method for isolating extraembryonic membranes from pregnant mice.

Isolation of Mouse Embryos Video Clip Isolation of mouse embryos video clip.

Making Holding Pipettes Protocol Protocol describes a method for making holding pipettes, to be used for microinjection.

Making Injection Pipettes Protocol Protocol describes the determination of useful settings with the Sutter Puller P-97 to make injection pipettes for microinjection.

Making Pipettes From Hard Glass Capillaries Protocol Protocol describes a method for making pipettes from hard glass capillaries.

Mouse Embryo Transfer into Pseudopregnant Recipient Protocol 1-cell embryo transfer into pseudopregnant recipient female mouse.

Planning an Effective Mouse Colony Considerations in planning an effective mouse colony for transgenic production. Includes: Mouse strain; Colony size; Efficiency of superovulation; Parental Suitability; Pseudo-pregnancy.

Preparing Siliconized Pipettes Protocol Protocol describes a method for preparing siliconized pipettes. Such pipettes minimize the loss of embryos or embryonic tissues during transfer.

Sectioning Mouse Embryos Protocol Protocol provides methods and tips for sectioning mouse embryos and transferring the sections to a microscope slide.

Transfer of Injected Blastocysts to Pseudo Pregnant Mice Protocol Blastocyst transfer is usually performed 24 hours after aggregation, when the morulae have become expanded blastocysts, and on the same day as injection. A little time is given between injection and transfer to allow the blastocysts to re-expand. Includes: The Mouse Recipient; The Transfer.

Whole Mount In Situ Hybridization of Mouse Protocol Protocol for whole mount in situ hybridization of mouse. Incluses: Preparation of the probe; General Comments; Preparation of embryos; In Situ hybridization.