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Molecular Biology Techniques

At Molecular Station Molecular Biology Techniques, you will find all the Molecular Biology Techniques listed alphabetically. Theory behind the technique, and protocols to conduct the technique are included below. Updated July 2011

For links to protocols used by other labs see our Protocols Directory

Basic Lab Protocols

Preparing Solutions in the Lab

Arabidopsis and Botany Protocols

Protoplast Isolation Protocol - for the isolation of Metabolically Competent Protoplasts. (Saji Menon and Vibha Tyagi)

Cell Biology

FACS Cell Sorting and Flow Cytometry


DNA Cloning

Agarose DNA Gel Electrophoresis

cDNA Synthesis

DNA Gel Electrophoresis

Membrane Hybridization Screening of Libraries

Maxam-Gilbert Sequencing

Gene Cloning

Restriction Enzyme Digestion

Genomic DNA Isolation Protocol

Baceteria DNA Isolation

DNA Transformation - Contains History of DNA

Electrophoretic Mobility Shift Assay

Yeast Two Hybrid Assay

Southern Blotting Technique and Method

Single Nucleotide Polymorphisms


Northern Blot

RNA Isolation

RNA Gel Electrophoresis

DEPC usage with RNA.

In Vitro Transcription protocol


Gel Electrophoresis

Autoclave Operation

Liquid Scintillation Counting


Non-radioactive detection methods and tracers.

HPLC - High Performance Liquid Chromotography


Immunoprecipitation Background

Immunoprecipitation Protocol

Mass Spectrometry

Mass Spectrometery

Edman Degradation

Electrospray MS


Protein Microarray Theory

PCR - Polymerase Chain Reaction

Theory and Development of PCR

Avoiding PCR Contamination

PCR Primer Design Guidelines and Software

Site Directed Mutagenesis

Protein Techniques

Protein Identification

Protein Purification

Protein Separation

Western Blot

Immunoprecipitation IP

Proteomics Methods

Recombinant Protein Expression

Recombinant Protein Expression

RNAi and SiRNA

SiRNA Transfection Protocol

SDS-PAGE Gel Electrophoresis

SDS-PAGE Gel Electrophoresis Background Theory

Trypsin Digestion (In Gel Trypsin Digestion)

In Gel Trypsin Digestion Theory

Transgenic Plants and Animals

Transgenic Animals

Western Blot

Western Blot Background Theory

Western Blot Troubleshooting

Nitrocellulose Filter Binding Assay

Western Blot Protocol

X-Ray Crystallography

X-ray Crystallography Background


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