At Molecular Station Molecular Biology Techniques, you will find all the Molecular Biology Techniques listed alphabetically. Theory behind the technique, and protocols to conduct the technique are included below. Updated July 2011
For links to protocols used by other labs see our Protocols Directory
[help] preparation of reagent[35]Sulfur Radiation Safetyyeast transportationYeast one hybridYeast Gene DisruptionYeast 2 Hybrid (didn't know where to post this)yeastY2H intrigueWowworking out a substrate concentrationWill MTT interfere with DNA assays ?will i get 75 to 80 percent scholarship for a GRE score of 1400 to 1500???Why treat plasmid DNA with alkaline phosphatase when making a recombinant plasmid?Why is DNA purification important?Why iam not able to get a band from my Plasmid DNA samples?
Preparing Solutions in the Lab
Protoplast Isolation Protocol - for the isolation of Metabolically Competent Protoplasts. (Saji Menon and Vibha Tyagi)
FACS Cell Sorting and Flow Cytometry
DNA Cloning
Agarose DNA Gel Electrophoresis
cDNA Synthesis
DNA Gel Electrophoresis
Membrane Hybridization Screening of Libraries
Maxam-Gilbert Sequencing
Gene Cloning
Restriction Enzyme Digestion
Genomic DNA Isolation Protocol
Baceteria DNA Isolation
DNA Transformation - Contains History of DNA
Electrophoretic Mobility Shift Assay
Yeast Two Hybrid Assay
Southern Blotting Technique and Method
Single Nucleotide Polymorphisms
Northern Blot
RNA Isolation
RNA Gel Electrophoresis
DEPC usage with RNA.
In Vitro Transcription protocol
Gel Electrophoresis
Autoclave Operation
Liquid Scintillation Counting
Phosphorimaging
Non-radioactive detection methods and tracers.
HPLC - High Performance Liquid Chromotography
Immunoprecipitation Background
Immunoprecipitation Protocol
Mass Spectrometery
Edman Degradation
Electrospray MS
Protein Microarray Theory
Theory and Development of PCR
Avoiding PCR Contamination
PCR Primer Design Guidelines and Software
Site Directed Mutagenesis
Protein Identification
Protein Purification
Protein Separation
Western Blot
Immunoprecipitation IP
Proteomics Methods
Recombinant Protein Expression
SiRNA Transfection Protocol
SDS-PAGE Gel Electrophoresis Background Theory
In Gel Trypsin Digestion Theory
Transgenic Animals
Western Blot Background Theory
Western Blot Troubleshooting
Nitrocellulose Filter Binding Assay
Western Blot Protocol
X-ray Crystallography Background
All Protocols
DNA Protocols - DNA Cloning
Cell Biology Protocols - Cell Culture Protocols
PCR Protocols Protein Protocols
RNAi Protocols
Western Blot Protocols
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