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Rapid DNA Ligation

Written by Super User. Posted in DNA

Learn about rapid DNA ligation.

Copyright 2014 Molecular Station

If you want quick, fast or rapid methods of DNA ligation for your cloning then you can use the:

Quick DNA Ligation Protocol

  • Use 8 ul of insert and vector (less than 200 ng)
  • It is suggested by BMC to use 3:1 vector to insert ratio if vector is de-phosphorylated
  • Use 2 ul of 5x DNA dilution buffer (Tube #1)
  • Add 10 ul of 2X ligation buffer (Tube #2)
  • Add 1 ul of ligase (assume you have to use "special ligase" Tube #3)
  • 5 min at room temp

Special Rapid DNA Ligases Include:

NEB's Quick Ligation Kit (5 Minute ligation cloning)

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