FM 4-64 Labeling of Yeast Vacuole Membranes Protocol FM 4-64 is a lipophilic styryl dye and a vital stain: it fluoresces only in living cells, so cells cannot be fixed then stained nor stained then fixed. You must stain and observe living cells. FM 4-64 does not permeate cell membranes but, instead, intercalates into the plasma membrane is then
taken into the cells by endocytosis.
Imaging, Measuring and Manipulating Native Biomolecular Systems with the Atomic Force Microscope The atomic force microscope (AFM) is one of the most powerful tools for determining the surface topography of native biomolecules at subnanometer resolution. The AFM can also provide insight into the binding properties of biological systems. In order to determine the specific interaction between two kinds of molecules (e.g., avidin and biotin). Includes information on principle of AFM and application of AFM.
Measuring Volume Protocol Protocol for measuring volume. Material required includes: Microscope, Hemacytometer and coverslip, Suspension of yeast.
Reflected Light Microscopy Reflected light microscopy is often referred to as incident light, epi-illumination, or metallurgical microscopy, and is the method of choice for fluorescence and for imaging specimens that remain opaque even when ground to a thickness of 30 microns.