Microarray Analysis Protocols

Anatomy of a Comparative Gene Expression Study Information on anatomy of a comparative gene expression study.  Includes: Choosing cell populations; mRNA Extraction and Reverse Transcription; Fluorescent labeling of cDNA's; Hybridization of DNA microarray; Scanning the hybridized array; Interpreting the scanned image.

aRNA Labeling and Hybridization Protocol Protocol for aRNA labeling and hybridization.

Genome-Wide Analysis Protocol Genome-wide analysis of data generated on the Affymetrix 10K Xba 142 arrays for identification of regions with high probability to contain genes responsible for Micronodular (non-pigmented) Adrenocortical Hyperplasia.

Isolation of Total RNA From E. Coli for Microarrays Protocol Protocol for isolation of total RNA from E. coli for microarrays. Includes: Harvesting total RNA; Dnase I treatment of RNA samples.

Microarray Analysis of Highly Purified Human Decidual NK Cells Protocol Description of gene array analysis and normalization

Microarray Protocols Protocols for microarrays. Includes: RNA amplification by in vitro transcription; cDNA labeling; Hybridization of cDNA microarrays.

Preparation of B-Lymphocyte RNA for Microarray Analysis Protocol Protocol permits the isolation of at least 5 µg of total RNA from a sample of purified mouse splenic B lymphocytes Isolation of Resting B Lymphocytes from Sixteen Mouse Spleens.

Preparation of Myocyte RNA for Microarray Analysis Protocol Protocol permits the isolation of at least 3 µg of total RNA from approximately 150,000 cultured cardiac myocytes from adult mice. Includes: Treatment of Samples and Termination of Reactions; Isolation of RNA; Use of Environmental Chamber.