Deamidation in Proteins and Peptides Deamidation in Proteins and Peptides. Informational page by Glen Teshima. RPLC, HIC, Peptide mapping of proteolytic digests (LC-MS), Protein carboxyl methyltransferase (PIMT).
Dried droplet Sample Preparation for MALDI Dried droplet Sample Preparation for MALDI.The discovery of this method allowed the application of laser desorption to proteins . Drying a droplet of a protein/matrix solution remains the favorite method of most MALDI practitioners. Protocol for dried
MALDI Matrices MALDI Matrices. Commonly used MALDI matrices for analysis of peptides, proteins, carbohydrates, and nucleic acids using 337 nm or 355 nm UV lasers. All matrices can be used for sample preparation using the Dried Droplet Method whereas only matrices solubl
Millipore's ZipTip Protocol Millipore's ZipTip Protocol. Intended for purifying and concentrating femtomoles to picomoles of protein, peptide or oligonucleotide samples prior to analysis, providing better data quality. Millipore.
Proteomic protocols and Peptide Extraction Colloidal coomassie blue staining, Destaining, Reduction/Alkylation, Peptide extraction For MALDI-MS analysis and For MALDI-MS analysis protocols and methods. Institut Curie Paris.
Protocols for Mass Spectrometry Analysis Preparation Protocols for Mass Spectrometry Analysis Preparation. Preparing gel slices for PMF or MS/MS , mass spec data interpretation, Submitting proteins in solution, Interpretation of LC-MS/MS data. BMS MASS SPECTROMETRY AND PROTEOMICS FACILITY
Seeded films (Vorm-Roepstorff) MALDI Protocol This sample preparation method is a direct replacement for the dried droplet technique. It produces more uniform samples than the dried-droplet technique and the crystals are better adhered to the sample stageâ€™s surface. MALDI Sample preparation. PROWL