MAPK Protocols

Activated MAP Kinase Protocols Stratagene PDF MAPK Protocols. Resolving Phosphorylated MAPK Proteins by SDS–PAGE, Filter Binding Assay for activated MAPK γ-32P incorporation of MAPK. Stratagene

Activation of p70s6k and MAP Kinases: IP and Kinase Activity Protocol describes the activation of MAP Kinase activity. Protocol contains information on: Buffers: ST, STE, Lysis Buffer Buffer A, Buffer B and 10x Kinase Buffer.

In Vitro MAP Kinase Assay Protocol describes an in vitro assay to detect MAP kinase activity using PC12 cells.

In-Vitro Kinase Assay for Jnk-1/IRS Proteins Protocol describing an in vitro assay on the detection of Kinase activity for Jnk-1/IRS proteins.

JNK Kinase Assay Protocol describing an JNK Kinase Assay. Protocol includes information on reaction buffer.

Kinase Assays Nature protocol entailing a kinase assay.

MAPK immunoprecipitation Protocol PDF MAPK immunoprecipitation protocol and methods. Upstate.

Mitogen Activated Protein Kinase Guide Mitogen Activated Protein Kinase Guide. MEK, MEKK, MAPK, MAPKK, antibodies and kits Promega.

Protocol for JNK Kinase Assay Protocol describing a JNK kinase asssay. Protocol includes information on: Triton Lysis Buffer, Kinase Assay Buffer, Kinase Assay and Preparation of kinase

Raf-1 Kinase Assay This protocol uses mitogen-activated protein/ERK kinase (MEK) to activate the extracellular-signal-regulated (Erk) mitogen-activated protein (MAP) kinases upon agonist binding to receptors. Protocol includes information about: Harvest, Immunoprecipitation and Kinase Reaction. Also includes following solutions: Laemmli Sample Buffer (1X), ATP Mix, Kinase Buffer (10X), Lysis Buffer. Helpful protocol hints are also included.

SAPK/Jun Kinase Assays Protocol for SAPK/Jun kinase assays. Protocol includes information on: preparation of cell lysate, substrate/antibody, lysate interaction.