Adapting Cells to a Serum-Free Environment Protocol There are many ways to adapt cell lines to serum-free media. Five methods are presented that are designed for adapting hybridomas to a protein-free medium. These protocols may require some modifications for your particular cell line and conditions.
Basic Techniques for Mammalian Cell Tissue Culture Protocol Cultured mammalian cells are used extensively in cell biology studies; it requires a number of special skills in order to be able to preserve the structure, function, behavior and biology of the cells. This unit describes the basic skills required to maintain and preserve cell cultures: aseptic technique, medium characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells.
General Cell Culture Covers general things you need to know to get started in cell culture work. Includes: Sterile Technique; Growth of Your Cell Culture;
Generation of Growth Curve Protocol Generation of a growth curve can be useful in evaluating the growth characteristics of a cell line.
From a growth curve, the lag time, population doubling time, and saturation density can be
Human Oesophageal Culture Protocol This procedure describes a method for establishing short-term explant cultures of oesophageal mucosa. Adverse effects produced by exposure to radiation or test compounds can be detected as an inhibition of cell outgrowth.
Human Thyroid Culture Protocol This method enables the culturing of thyroid cells without loss of differentiation and medium change. It is potentially useful for the long-term study of drug effects on the thyroid gland.
IMEC Maintenance Information on Immortalized Mammary Epithelial Cells (IMEC). Includes: Background Information on IMEC; Culturing; Trypsinization.
Isolation of Adult Mouse Cardiac Myocytes from Two or More Hearts Protocol This procedure describes the isolation and culture of adult mouse cardiac myocytes from two or more hearts. Includes
modifications for the digestion of two or more hearts in the same procedure and subsequent pooling of myocytes derived from the multiple hearts. The isolation procedure is performed by one or more technicians and routinely yields approximately 1
million rod-shaped myocytes per heart.
Protocol for Isolation, Cryopreservation, and Thawing of PBMCs Cryopreserved PBMCs are a common specimen source for studies of immunological responses to vaccines,
immunotherapies, etc. The health and viability of cells recovered post-cryopreservation are of course
critical to the success and accuracy of immunological assays performed on them. This protocol standardizes PBMC isolation and cryopreservation techniques, specifically for the
assessment of thawed cells by cytokine flow cytometry.
Quality Control Considerations for Cell Culture Quality control considerations for cell culture. Includes: Provenance and Integrity of Cell Lines; Avoidance of Microbial Contamination; Environmental Monitoring; What to do in the event of contamination;