Insect Cell Culture Protocols

Adaptation of High Five™ Cells to HyQ© SFX Insect Medium This protocol is designed to adapt High Five™ (BTI-Tn-4) cells to serum-free suspension culture in HyQ SFX-Insect in 5 passages. The entire adaptation process takes approximately two weeks and the resulting adapted cells have doubling times of 16 and 20 hours and minimal clumping in suspension culture.

Baculovirus Protein Expression Protocols Baculovirus protein expression protocols. Includes protocols: Cotransfection Protocol; Analysis of nonsecreted protein expression; Growing and Freezing Insect Cells.

Culturing of Insect Cells and Production of Baculovirus Expressed Recombinant Proteins in 2.8 Liter The use of Ferbach flasks is an ideal method to amplify the cells and virus inoculum for the bioreactor. The method is also very practical for producing sufficient amounts of various proteins for feasibility studies using HyQ© SFX-Insect™ medium. The large surface area in this vessel enables sufficient aeration and, therefore, the cells, when they reach high density, are not oxygen depleted.

Direct Adaptation of Insect Cells to HyQ© SFX-Insect™ Medium Using this method, insect cells should be adapted to HyQ SFX-Insect in 4-8 passages. However, there are some cell lines and clones that are more sensitive to the physiochemical and nutritional changes, and in some cases it may take longer than 8 passages for the adaptation.

Insect Cell Culture and Baculovirus Infections Protocol Protocol for insect cell culture and baculovirus infections. Includes: Cells, Media, Growth Characteristics, Freezing and Thawing Cells, and Glassware.

Methods for Maintaining Insect Cell Cultures Insect cell cultures are now commonly used in insect physiology, developmental biology, pathology, and molecular biology. As the field has advanced from methods development to a standard procedure, so has the diversity of scientists using the technique. This paper describes methods that are effective for maintaining various insect cell lines. The procedures are differentiated between loosely or non-attached cell strains, attached cell strains, and strongly adherent cell strains.

Passage of Adherent Cell Lines (subculture). Remove medium from culture container, rinse with PBS, expose cells to trypsin, incubate, stop trypsin activity by adding serum containing medium, dissociate cells, dilute and return to incubator.

Production of Autographa californica Multiple Capsid Nuclear Polyhedrosis Virus (rAcMNPV) Stock Production of Autographa californica Multiple Capsid Nuclear Polyhedrosis Virus (rAcMNPV) Stock.

Production of Recombinant Protein Using the Baculovirus Expression Vector System (BEVS) Production of recombinant protein using the baculovirus expression vector system (BEVS).

Protocols for Insect Cell Culture Includes protocols: Direct Adaptation to HyQ© SFX-Insect™ Medium; Sequential Adaptation to HyQ© SFX-Insect™ Medium; Adaptation of High Five™ Cells to HyQ© SFX-Insect™; Production of Recombinant Protein Using the Baculovirus Expression Vector System; Culturing Insect Cells and Production of Baculovirus Expressed Proteins in 2.8 L Fernbach Systems. Production of Autographa californica Multiple Capsid Nuclear Polyhedrosis Virus (rAcMNPV) Stock

Sequential Adaptation to HyQ© SFX-Insect™ Medium Protocol Protocol for sequential adaptation to HyQ© SFX-insect™ medium.

Thawing & Freezing Protocols for Insect Cells (BV) Protocols for thawing and freezing insect cells (BV).