Immunoaffinity Chromatography Protocols

Affinity Antibody Purification of Protein A/G Chromatography Antibody Purification (Antiserum or Ascites by Protein A/G Chromatography). Species and Type of Antibody Agarose Rabbit IgG Protein A or Protein G Mouse IgG1 Protein G Mouse IgG2 Protein A or Protein G Mouse IgG3 Protein G Sheep IgG Protein G but binds weakly Rat IgG Protein G but binds weakly Guinea Pig IgG Protein A Dog IgG Protein A Goat IgG Protein G Pig IgG Protein A Hamster IgG Protein G. By Millipore.

ANTIBODY PURIFICATION by affinity chromatography ANTIBODY PURIFICATION by affinity chromatography. By Beth, Mullins Lab UCSF. To affinity purify antibodies, generate lots of E. coli lysate that contains your antigen. If the protein can stand freeze thawing, then go ahead and purify the protein from e. coli lysate and keep it frozen until you need to couple it to a CH-sepharose column.

Directed Orientation of Antibodies during Preparation of Antibody Affinity Resin Protocol Protocol describes the directed coupling of antibody to protein G-agarose via its Fc domain, and subsequent covalent cross-linking of the complex using dimethyl pimelimidate (DMP).

Enzyme Immunoaffinity Chromatography—A Rapid Semi-quantitative Method Enzyme Immunoaffinity Chromatography—A Rapid Semi-quantitative Method.

Immunoaffinity Purification Gibberellins - PDF Immunoaffinity Purification Gibberellins. PDF.

Immunoaffinity Purification: Coupling Antibodies to Protein A or G Bead Columns Protocol Protocol describes how antibodies are bound to protein A or protein G beads and are then crosslinked to the matrix via a bifunctional coupling reagent called dimethyl pimelimidate (DMP).

Immunoaffinity Purification: Eluting Antigens from Immunoaffinity Columns Protocol This elution procedure relies on disrupting the bonds between the antibody and the antigen.

Purification of Antibodies on an Antigen Column Protocol Immunoaffinity purification of antibodies is used to purify antigen-specific antibodies from a preparation of polyclonal antibodies.  Such purification is commonly needed in the production of antipeptide antibodies, where it is used to concentrate the desired antibodies and separate them from those raised against carrier proteins.  It is also used for the more general purpose of removing unwanted, nonspecific binding activity from polyclonal antibody preparations.

Purification of the Adipocyte Insulin Receptor by Immunoaffinity Chromatography Purification of the Adipocyte Insulin Receptor by Immunoaffinity Chromatography.

Removal of Cross-reactive Antibodies from Antiserum: Affinity Chromatography Protocol Protocol describes a method for removing antibodies that react with bacterially encoded proteins by passing a crude preparation of immunoglobulins through a column containing immobilized bacterial proteins.