Immobilized Metal Ion Affinity Chromatography Protocols Protocol Links
Chelating Sepharose Fast Flow Protocol Immobilized metal ion affinity chromatography (IMAC) exploits a molecule’s affinity for chelated metal ions. The amino acid histidine present in many proteins forms complexes with transition metal ions such as Cu2+, Zn2+, Ni2+ and Fe3+. Chelating Sepharose™ Fast Flow with a suitable immobilized metal ion will therefore selectively retain proteins with exposed histidine.
Construction of Micro-Tip for Use in IMAC Protocol Protocol is the second step in a three-step process for the preparation and enrichment of phosphopeptides using immobilized metal affinity chromatography (IMAC) for the identification of the phosphopeptides by LCMS/ MS. This procedure describes the construction of microchromatographic columns, or micro-tips.
Lysis and Protein Extraction from WEHI-231 Cells with TriPure Isolation Reagent Protocol Protocol is the first step in a three-step process for the preparation and enrichment of phosphopeptides using immobilized metal affinity chromatography (IMAC) for the identification of the phosphopeptides by LC-MS/MS. This procedure is used to prepare protein extracts from WEHI-231 cells. This preparation method provides total cellular protein samples that are free of contaminating nucleic acids.
Packing an IMAC Column Protocol Protocol for packing an IMAC column. Protocol describes a procedure for packing a 30-ml immobilized metal-ion affinity chromatography (IMAC) column with the aid of a pump.
Preparation and Enrichment of Phosphopeptides from Phosphotyrosine Protocol Protocol is specifically for the further enrichment of phosphopeptides from a phosphotyrosine pull-down. This is the final step for the preparation and enrichment of phosphopeptides using immobilized metal affinity chromatography (IMAC) for the identification of the phosphopeptides by liquid chromatography tandem mass spectrometry (LC-MS/MS).
Preparation of a Prepacked IMAC Column Protocol Protocol for the preparation of a prepacked IMAC column. Purification of histidine-tagged proteins on a milligram scale can be performed conveniently on a small (1to 5-ml) immobilized metal-ion chromatography (IMAC) column using a syringe to load the sample, wash the column, and elute the protein.
Purification of Histidine-Tagged Proteins under Denaturing Conditions Using IMAC Protocol Immobilized metal-ion affinity chromatography (IMAC) is suitable for the purification of proteins under denaturing conditions. Either guanidine-HCl or urea can be used, although guanidine-HCl is a stronger denaturant than urea. Proteins that have been adsorbed to the column in the presence of guanidine-binding buffer may be washed with urea-binding buffer and eluted with urea elution buffer.
C and M Law Corporation's
Los Angeles personal injury attorney firm has been serving the city’s residents for over 45 years. People who think they do not need the services of an experienced personal injury attorney, invariably find out the hard way that they should have chosen that right lawyer in the very beginning. Regardless of the type of accident or injury, we have the experience to successfully represent you and your family. If you or someone you know has been injured through the negligence or recklessness of others, come see us. Voted in the top one percent of trial lawyers in the USA, we can help get you the compensation you and your loved ones deserve. The personal injury attorney Los Angeles firm of C and M Law Corporation has won an excess of 2 Billion Dollars in settlements!