Linkage Analysis Using the NaOH Methylation Method Protocol Linkage analysis provides information on sugar type, ring size, and substitution positions for each monosaccharide. The method in this protocol, using NaOH as the base, is one of the simpler linkage analysis methods. It requires approximately 1-5 µg of carbohydrate.
Monosaccharide Analysis by Methanolysis Protocol Protocol describes monosaccharide analysis by methanolysis. The method gives more complex chromatograms than the corresponding alditol acetate method, because up to four methyl glycosides can be produced from each monosaccharide. However, the method is easier to perform.
Monosaccharide Analysis of Phosphorylated Sugars by Methanolysis and Diazomethane Methylation Protocol presents a method that allows rapid determination of the monosaccharide composition of glycans, glycoproteins, and proteoglycans that contain (or are suspected to contain) phosphorylated sugars. (For samples that do not contain phosphorylated sugars see Monosaccharide Analysis by Methanolysis.)
Monosaccharide Composition Analysis: Alditol Acetates Protocol Protocol describes how to determine the monosaccharide composition of glycans, glycoproteins, or proteoglycans by hydrolyzing the sample to monosaccharides and converting them to alditols, then performing acetylation to make them volatile compounds and analysis by gas chromatography (GC) or gas chromatography coupled with mass spectrometry (GC-MS).
