Bioassay for Determining Itraconazole Levels in Blood Serum concentrations of itraconazole should be measured in patients receiving this drug to ensure that therapeutic concentrations are being achieved. This is necessary as drug absorption can be variable, and levels may be lowered by interactions with other drugs. The assay will give an indication of whether suitable blood levels have been achieved.
Phagocytosis Assay Protocol Protocol for phagocytosis assay. Protocol describes how to assess the efficiency of phagocytic cells to engulf conidia of a given fungus.
Preparation of Solid Tissue for Aspergillus Galactomannan Antigen Detection by Platelia Protocol Protocol for the preparation of solid tissue for Aspergillus galactomannan antigen detection by Platelia (Biorad). Technique was designed for use on human serum. However, it may also be possible to perform this method on solid tissues and organic solutions. Viscous solution and tissue specimens need to be pre-treated to achieve the extraction of the Aspergillus antigen and to get a homogeneous sample in solution.