Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > DNA Techniques > DNA Extraction Forum
Register Search Today's Posts Mark Forums Read

DNA Extraction Forum DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.


PBS buffer

PBS buffer - DNA Extraction Forum

PBS buffer - DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-12-2011, 11:14 PM
Pipette Filler
Points: 289, Level: 5 Points: 289, Level: 5 Points: 289, Level: 5
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2010
Posts: 6
Thanks: 1
Thanked 3 Times in 1 Post
Default PBS buffer



I need to prepare 100 ml of PBS, pH 7.2 that contains 50 mM potassium phosphate and 150 mM NaCl. Can anyone help me with the calculations?
Thanks.
Reply With Quote
  #2  
Old 01-14-2011, 07:55 PM
luisillo's Avatar
M.D/Ph.D
Points: 4,149, Level: 43 Points: 4,149, Level: 43 Points: 4,149, Level: 43
Activity: 33% Activity: 33% Activity: 33%
 
Join Date: Jul 2010
Location: Mexico
Posts: 354
Thanks: 20
Thanked 98 Times in 90 Posts
Default Re: PBS buffer

-Potassium phosphate

KH2PO4: MW= 136.09

1 M = 136.09 g/L
1 mM = 0.13609 g/L
50 mM = 6.8045 g/L

-Sodium chloride

NaCl: MW= 58.443

1 M = 58.443 g/L
1 mM = 0.058443 g/L
150 mM = 8.76645 g/L

Hope it helps you
Reply With Quote
The Following 2 Users Say Thank You to luisillo For This Useful Post:
admin (01-14-2011), Nikola (01-16-2011)
Reply

Tags
buffer , pbs


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
protein purification magnificientleo Proteomics Forum 11 12-29-2010 09:01 AM
SDS-PAGE Protocol moleculardude Protein Science 14 02-12-2010 11:27 AM
Production of Monoclonal Antibodies - Technique in Mouse Cellbiogal Antibody Forum 2 01-08-2009 10:52 PM
Methods Digest, Vol 18, Issue 19 1. Qiagen buffer PN Jess Protocols and Methods Forum 0 11-22-2006 10:45 PM
Qiagen buffer PN Daniel Ciznadija Protocols and Methods Forum 0 11-19-2006 10:12 PM


All times are GMT. The time now is 03:37 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12256 seconds with 16 queries