Detection FISH Protocol Protocol for Detection (FISH). TRITC, or Avidin Cy-5. For the probes labeled with digoxigenin, we usually first incubate with mouse-anti-digoxigenin, followed by incubation with sheep anti-mouse Cy5.5, or other fluorochrome conjugated antibodies.
FISH Guide and Troubleshooting FISH Guide and Troubleshooting. Includes: Cytogenetic techniques; Slide preparation; When do chromosomes spread; Cell suspensions; G-banding; Cell fixatives; Slide preparation factors.
Fluorescence In Situ Hybridization (FISH) for DNA Replication Origins Protocol Protocol for fluorescence in situ hybridization (FISH) for DNA replication origins. Fluorescent in situ hybridization (FISH) is a molecular cytogenetic technique used for the detection of specific chromosomal rearrangements and applicable to many different specimen types. FISH is widely used for several diagnostic applications.
Fluorescence In Situ Hybridization of a Repetitive DNA Probe to Human Chromosomes in Suspension Protocol for fluorescence in situ hybridization of a repetitive DNA probe to human chromosomes in suspension. Hybridization technique which does not need formamide and dextran sulfate. As a model system, we used the repetitive
specific human DNA probe pUC 1.77, labeled it with digoxigenin-11-dUTP by nick-translation, and hybridized it to
metaphase chromosomes in suspension. These chromosomes were isolated by standard techniques from human lymphocytes.
Fluorescent In Situ Transcription in Cells and Tissues Protocol Method assesses cellular mRNA transcripts in tissue sections and cell cultures using unique short anti-sense primers directed against sequences in particular protein(s). The unlabeled synthetic cDNA oligonucleotide primers are extended complementary to a sense mRNA transcript using reverse transcriptase and labeled through incorporation of a fluorescent-labeled dUTP nucleotide base. This procedure provides rapid detection of low abundance mRNA messages that can be related to other cellular....
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