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SDS-PAGE GEL CALCULATOR

Automatic Calculations for SDS Polyacrylamide Gel Recipe

Select the number of Gels required (for BIORAD ie. 18x16mm / Mini-Protean protein) with drop down (leave default if need only 1 gel)

Enter the Number of Gels Needed (#):
 

Enter the SEPARATING GEL % (percentage ie 7-12%+) polyacrylamide required

Enter Separating Gel Percent Required:
%

MATERIALS AND BUFFER VOLUMES REQUIRED BELOW:

 

Buffer Volumes

SEPARATING Gel

 
ml ddH2O
 
ml Acrylamide
 
ml 1.5M Tris pH 8.8
 
µl 10% SDS
 
µl 10% APS
 
µl TEMED
 
ml TOTAL VOLUME

 

 

Buffer Volumes

STACKING Gel

 
ml ddH2O
 
ml % Acrylamide
 
ml 0.5M Tris pH 6.8
 
µl 10% SDS
 
µl 10% APS
 
µl TEMED
 
ml TOTAL VOLUME
  SELECT STACKING GEL % :   Stacking gel
       

 

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