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Visualizing Smad1/4 signaling response to bone morphogenetic Protein-4 activation by FRET biosensors.

Visualizing Smad1/4 signaling response to bone morphogenetic Protein-4 activation by FRET biosensors. Research Abstract Details 

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  • Visualizing Smad1/4 signaling response to bone morphogenetic Protein-4 activation by FRET biosensors. Abstract Text:

    kira v gromovaKira V Gromova,mike friedrichMike Friedrich,andrey noskovAndrey Noskov,gregory s harmsGregory S Harms,kira v gromovaKira V Gromova,mike friedrichMike Friedrich,andrey noskovAndrey Noskov,gregory s harmsGregory S Harms,kira v gromovaKira V Gromova,mike friedrichMike Friedrich,andrey noskovAndrey Noskov,gregory s harmsGregory S Harms,

    Smad proteins are the major signal transducers for the Transforming Growth Factor superfamily of cytokines and their serine/threonine kinase receptors. Smads mediate the signal from the membrane into the nucleus. Bone Morphogenetic Protein-4 stimulates phosphorylation of Smad1, which interacts with Smad4. This complex translocates into the nucleus and regulates transcription of target genes. Here, we report our development of cellular fluorescence biosensors for direct visualization of Smad signaling in live mammalian cells. Fluorescence resonance energy transfer between cyan and yellow fluorescent proteins fused to the Smad1 and Smad4 proteins was used to unravel the temporal aspects of BMP/Smad signaling. A rate-limiting delay of 2-5 min occurred between BMP activation and Smad1 activity. A similar delay was observed in the Smad1/Smad4 complexation. Further experimentation indicated that the delay is dependent on the MH1 domain and linker of Smad1. These results give new insights into the dynamics of the BMP receptor -Smad1/4 signaling process and provide a new tool for studying Smads.

    Visualizing Smad1/4 signaling response to bone morphogenetic Protein-4 activation by FRET biosensors. Publishing Authors By Initials

    kv gromovaKV Gromova,m friedrichM Friedrich,a noskovA Noskov,gs harmsGS Harms,kv gromovaKV Gromova,m friedrichM Friedrich,a noskovA Noskov,gs harmsGS Harms,kv gromovaKV Gromova,m friedrichM Friedrich,a noskovA Noskov,gs harmsGS Harms,

    For similar abstracts research abstracts see: abstracts research

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    Visualizing Smad1/4 signaling response to bone morphogenetic Protein-4 activation by FRET biosensors. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Biochimica et biophysica acta

    VOLUME: 1773

    Page Numbers: 1759-73

    Journal Abbreviation: Biochim. Biophys. Acta

    ISSN: 0006-3002

    DAY: 9

    MONTH: 10

    YEAR: 2007

    Visualizing Smad1/4 signaling response to bone morphogenetic Protein-4 activation by FRET biosensors. Information

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    LANGUAGE: eng

    NlmUniqueID: 217513

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    Grant and Affiliation Information for Visualizing Smad1/4 signaling response to bone morphogenetic Protein-4 activation by FRET biosensors.

    AFFILIATION: Molecular Microscopy Group, Rudolf-Virchow-Center, University of Würzburg, Versbacher Str. 9, D-97078 Würzburg, Germany.

    Country: Netherlands

    Netherlands Research PublicationNetherlands Research Publication

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    MEDLINETA: Biochim Biophys Acta

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