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Ubp43 gene expression is required for normal Isg15 expression and fetal development.

Ubp43 gene expression is required for normal Isg15 expression and fetal development. Research Abstract Details 

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  • Ubp43 gene expression is required for normal Isg15 expression and fetal development. Abstract Text:

    lea a rempelLea A Rempel,kathleen j austinKathleen J Austin,kenneth j ritchieKenneth J Ritchie,ming yanMing Yan,meifeng shenMeifeng Shen,dong-er zhangDong-Er Zhang,luiz e henkesLuiz E Henkes,thomas r hansenThomas R Hansen,

    BACKGROUND: Isg15 covalently modifies murine endometrial proteins in response to early pregnancy. Isg15 can also be severed from targeted proteins by a specific protease called Ubp43 (Usp18). Mice lacking Ubp43 (null) form increased conjugated Isg15 in response to interferon. The Isg15 system has not been examined in chorioallantoic placenta (CP) or mesometrial (MM) components of implantation sites beyond 9.5 days post coitum (dpc). It was hypothesized that deletion of Ubp43 would cause disregulation of Isg15 in implantation sites, and that this would affect pregnancy rates. METHODS: Heterozygous (het) Ubp43 mice were mated and MM and CP implantation sites were collected on 12.5 and 17.5 days post-coitum (dpc). RESULTS: Free and conjugated Isg15 were greater on 12.5 versus 17.5 dpc in MM. Free and conjugated Isg15 were also present in CP, but did not differ due to genotype on 12.5 dpc. However, null CP had greater free and conjugated Isg15 when compared to het/wt on 17.5 dpc. Null progeny died in utero with fetal genotype ratios (wt:het:null) of 2:5:1 on 12.5 and 2:2:1 on 17.5 dpc. Implantation sites were disrupted within the junctional zone and spongiotrophoblast, contained less vasculature based on lectin B4 staining and contained greater Isg15 mRNA and VEGF protein in Ubp43 null when compared to wt placenta. CONCLUSION: It is concluded that Isg15 and its conjugates are present in implantation sites during mid to late gestation and that deletion of Ubp43 causes an increase in free and conjugated Isg15 at the feto-maternal interface. Also, under mixed genetic background, deletion of Ubp43 results in fetal death.

    Ubp43 gene expression is required for normal Isg15 expression and fetal development. Publishing Authors By Initials

    la rempelLA Rempel,kj austinKJ Austin,kj ritchieKJ Ritchie,m yanM Yan,m shenM Shen,de zhangDE Zhang,le henkesLE Henkes,tr hansenTR Hansen,

    For similar proteins: ubiquitins research abstracts see: proteins: ubiquitins research

    PUBMED ID PMID:

    MEDLINE DATE:

    Ubp43 gene expression is required for normal Isg15 expression and fetal development. Journal Published:

    PUBLICATION TYPE: Research Support, N.I.H., Extr

    Journal: Reproductive biology and endocrinology : RB&E;

    VOLUME: 5

    Page Numbers: 13

    Journal Abbreviation:

    ISSN: 1477-7827

    DAY: 26

    MONTH: 03

    YEAR: 2007

    Ubp43 gene expression is required for normal Isg15 expression and fetal development. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 101153627

    Ubp43 gene expression is required for normal Isg15 expression and fetal development. Keywords Mesh Terms:

    KEYWORDS: Ubiquitins

    MESH TERMS: metabolism

    Chemical & Substance for Abstract: Ubp43 gene expression is required for normal Isg15 expression and fetal development. Information

    Substance Name: Usp18 protein, mouse

    Registry Number: EC 3.4.99.-

    Grant and Affiliation Information for Ubp43 gene expression is required for normal Isg15 expression and fetal development.

    AFFILIATION: Department of Animal Science, University of Wyoming, Laramie, Wyoming, 82071, USA. lrempel@kumc.ecu

    Country: England

    England Research Publication

    AGENCY: United States NCRR

    GRANT: P20 RR 016474

    ACRONYM: RR

    MEDLINETA: Reprod Biol Endocrinol

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    DATABASENAME:

    ACCESSION NUMBER:

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