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Time-dependent loss of radioimmunoassayable levels of progesterone following ambient temperature incubation of heparinized bovine blood.

Time-dependent loss of radioimmunoassayable levels of progesterone following ambient temperature incubation of heparinized bovine blood. Research Abstract Details 

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  • Time-dependent loss of radioimmunoassayable levels of progesterone following ambient temperature incubation of heparinized bovine blood. Abstract Text:

    r e owensR E Owens,d t atkinsD T Atkins,c h raheC H Rahe,j l fleegerJ L Fleeger,p g harmsP G Harms,

    Plasma progesterone levels in heparinized blood collected at 10 min intervals for 8 continuous hours from four nulliparous Holstein cows on day 3 (early luteal), day 10 or 11 (mid-luteal) and day 18 or 19 of the estrous cycle were found to decline over time when blood was incubated at ambient temperature. The loss was more obvious during the mid-luteal collection period than either the day 3 or day 18 or 19 periods in all cows. This appeared to be associated more with high progesterone levels on day 10 or 11 rather than with differences in the period of the estrous cycle. There was an average decrease in progesterone levels of 3.4, 1.0 and 1.5 ng/ml between samples having the shortest and longest incubation periods on day 10 or 11, day 3 and day 18 or 19, respectively. This apparent decrease in levels of progesterone from bovine blood indicates need in the future for careful consideration concerning the handling of bovine blood collected for subsequent radioimmunoassay (RIA) of progesterone. Further work to elucidate the mechanism which is responsible for the apparent loss is also warranted.

    Time-dependent loss of radioimmunoassayable levels of progesterone following ambient temperature incubation of heparinized bovine blood. Publishing Authors By Initials

    re owensRE Owens,dt atkinsDT Atkins,ch raheCH Rahe,jl fleegerJL Fleeger,pg harmsPG Harms,

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    Time-dependent loss of radioimmunoassayable levels of progesterone following ambient temperature incubation of heparinized bovine blood. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Theriogenology

    VOLUME: 13

    Page Numbers: 305-9

    Journal Abbreviation: Theriogenology

    ISSN: 0093-691X

    DAY: 26

    MONTH: Apr

    YEAR: 1980

    Time-dependent loss of radioimmunoassayable levels of progesterone following ambient temperature incubation of heparinized bovine blood. Information

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    LANGUAGE: eng

    NlmUniqueID: 421510

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    Grant and Affiliation Information for Time-dependent loss of radioimmunoassayable levels of progesterone following ambient temperature incubation of heparinized bovine blood.

    AFFILIATION: Animal Science Department Texas Agricultural Experiment Station Texas A&M University College Station 77843 USA.

    Country: United States

    United States Research PublicationUnited States Research Publication

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    MEDLINETA: Theriogenology

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