Special Feature

User Panel

My Panel

My Panel

Bookmark Science Articles

Recent News
Bookmark / Share This Science Site

Stoichiometry of a pore mutation that abolishes picrotoxin-mediated antagonism of the GABAA receptor.

Stoichiometry of a pore mutation that abolishes picrotoxin-mediated antagonism of the GABAA receptor. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

  • Abstract Text of This Paper
  • Journal Published
  • MeSH Keywords of This Abstract
  • Chemicals and Substances Used in this Paper
  • Grants and Granting Agency of this Research
  • Database Accession Numbers Used in this Paper
  • Related Papers
  • Related Research Tags
  • Rate this Research Paper
  • Stoichiometry of a pore mutation that abolishes picrotoxin-mediated antagonism of the GABAA receptor. Abstract Text:

    anna sedelnikovaAnna Sedelnikova,brian e erkkilaBrian E Erkkila,holly harrisHolly Harris,stanislav o zakharkinStanislav O Zakharkin,david s weissDavid S Weiss,

    Picrotoxin, a potent antagonist of the inhibitory central nervous system GABAA and glycine receptors, is believed to interact with residues that line the central ion pore. These pore-lining residues are in the second transmembrane domain (TM2) of each of the five constituent subunits. One of these amino acids, a threonine at the 6' location, when mutated to phenylalanine, abolishes picrotoxin sensitivity. It has been suggested that this threonine, via hydrogen bonding, directly interacts with the picrotoxin molecule. We previously demonstrated that this mutation, in the alpha, beta or gamma subunit, can impart picrotoxin resistance to the GABA receptor. Since the functional pentameric GABA receptor contains two alpha subunits, two beta subunits and one gamma subunit, it is not clear how many alpha and beta subunits must carry this mutation to impart the resistant phenotype. In this study, by coexpression of mutant alpha or beta subunits with their wild-type counterparts in various defined ratios, we demonstrate that any single subunit carrying the 6' mutation imparts picrotoxin resistance. Implications of this finding in terms of the mechanism of antagonism are considered.

    Stoichiometry of a pore mutation that abolishes picrotoxin-mediated antagonism of the GABAA receptor. Publishing Authors By Initials

    a sedelnikovaA Sedelnikova,be erkkilaBE Erkkila,h harrisH Harris,so zakharkinSO Zakharkin,ds weissDS Weiss,

    For similar animals: chordata: vertebrates: amphibia: anura: pipidae: xenopus: xenopus laevis research abstracts see: animals: chordata: vertebrates: amphibia: anura: pipidae: xenopus: xenopus laevis research

    PUBMED ID PMID:

    MEDLINE DATE:

    Stoichiometry of a pore mutation that abolishes picrotoxin-mediated antagonism of the GABAA receptor. Journal Published:

    PUBLICATION TYPE: Research Support, N.I.H., Extr

    Journal: The Journal of physiology

    VOLUME: 577

    Page Numbers: 569-77

    Journal Abbreviation: J. Physiol. (Lond.)

    ISSN: 0022-3751

    DAY: 21

    MONTH: 09

    YEAR: 2006

    Stoichiometry of a pore mutation that abolishes picrotoxin-mediated antagonism of the GABAA receptor. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 266262

    Stoichiometry of a pore mutation that abolishes picrotoxin-mediated antagonism of the GABAA receptor. Keywords Mesh Terms:

    KEYWORDS: Xenopus laevis

    MESH TERMS: metabolism

    Chemical & Substance for Abstract: Stoichiometry of a pore mutation that abolishes picrotoxin-mediated antagonism of the GABAA receptor. Information

    Substance Name: Picrotoxin

    Registry Number: 124-87-8

    Grant and Affiliation Information for Stoichiometry of a pore mutation that abolishes picrotoxin-mediated antagonism of the GABAA receptor.

    AFFILIATION: Department of Physiology, UTHSCSA, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA.

    Country: England

    England Research Publication

    AGENCY: United States PHS

    GRANT: 35291

    ACRONYM:

    MEDLINETA: J Physiol

    REFSOURCE:

    DATABASENAME:

    ACCESSION NUMBER:

    Number Hits: 0

    Stoichiometry of a pore mutation that abolishes picrotoxin-mediated antagonism of the GABAA receptor Related Publications

     

    Molecular Station USER Menu

    Welcome to Molecular Station!

    You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!

    Already registered? Login now below.

    User Name:

    Password:

    Already registered and Forgot your password? Click below to recover it.

    Recover Lost Password

    Join now - it's fast and free!

    Molecular Station is THE largest network of researchers, scientists and science lovers anywhere!

    Research Terms of Usage and Disclaimer
    Home
    Features

    Protocols

    DNA Forum

    Science Forum

    DNA Forum
    Biology Forum

    Science News


    [CaRP] XML error: Invalid document end at line 2

    For more click here:Science News