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Shot-noise limited single-molecule FRET histograms: comparison between theory and experiments.

Shot-noise limited single-molecule FRET histograms: comparison between theory and experiments. Research Abstract Details 

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  • Shot-noise limited single-molecule FRET histograms: comparison between theory and experiments. Abstract Text:

    eyal nirEyal Nir,xavier michaletXavier Michalet,kambiz m hamadaniKambiz M Hamadani,ted a laurenceTed A Laurence,daniel neuhauserDaniel Neuhauser,yevgeniy kovchegovYevgeniy Kovchegov,shimon weissShimon Weiss,

    We describe a simple approach and present a straightforward numerical algorithm to compute the best fit shot-noise limited proximity ratio histogram (PRH) in single-molecule fluorescence resonant energy transfer diffusion experiments. The key ingredient is the use of the experimental burst size distribution, as obtained after burst search through the photon data streams. We show how the use of an alternated laser excitation scheme and a correspondingly optimized burst search algorithm eliminates several potential artifacts affecting the calculation of the best fit shot-noise limited PRH. This algorithm is tested extensively on simulations and simple experimental systems. We find that dsDNA data exhibit a wider PRH than expected from shot noise only and hypothetically account for it by assuming a small Gaussian distribution of distances with an average standard deviation of 1.6 A. Finally, we briefly mention the results of a future publication and illustrate them with a simple two-state model system (DNA hairpin), for which the kinetic transition rates between the open and closed conformations are extracted.

    Shot-noise limited single-molecule FRET histograms: comparison between theory and experiments. Publishing Authors By Initials

    e nirE Nir,x michaletX Michalet,km hamadaniKM Hamadani,ta laurenceTA Laurence,d neuhauserD Neuhauser,y kovchegovY Kovchegov,s weissS Weiss,

    For similar natural sciences: chemistry: chemistry, physical: phase transition research abstracts see: natural sciences: chemistry: chemistry, physical: phase transition research

    PUBMED ID PMID:

    MEDLINE DATE:

    Shot-noise limited single-molecule FRET histograms: comparison between theory and experiments. Journal Published:

    PUBLICATION TYPE: Research Support, U.S. Gov't,

    Journal: The journal of physical chemistry. B

    VOLUME: 110

    Page Numbers: 22103-24

    Journal Abbreviation:

    ISSN: 1520-6106

    DAY: 9

    MONTH: Nov

    YEAR: 2006

    Shot-noise limited single-molecule FRET histograms: comparison between theory and experiments. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 101157530

    Shot-noise limited single-molecule FRET histograms: comparison between theory and experiments. Keywords Mesh Terms:

    KEYWORDS: Phase Transition

    MESH TERMS: chemistry

    Chemical & Substance for Abstract: Shot-noise limited single-molecule FRET histograms: comparison between theory and experiments. Information

    Substance Name: DNA

    Registry Number: 9007-49-2

    Grant and Affiliation Information for Shot-noise limited single-molecule FRET histograms: comparison between theory and experiments.

    AFFILIATION: Department of Chemistry and Biochemistry, and California NanoScience Institute, University of California at Los Angeles, Los Angeles, California 90095, USA.

    Country: United States

    United States Research PublicationUnited States Research Publication

    AGENCY: United States NIGMS

    GRANT: 1R01-GM65382

    ACRONYM: GM

    MEDLINETA: J Phys Chem B

    REFSOURCE:

    DATABASENAME:

    ACCESSION NUMBER:

    Number Hits: 0

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