Role of protein supplements in the culture of mouse embryos.

Role of protein supplements in the culture of mouse embryos. Research Abstract Details 

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Role of protein supplements in the culture of mouse embryos. Abstract Text:

S Natsuyama,Y Noda,K Narimoto,T Mori,

The effect of various types of proteins used in single protein supplements for Bigger-Whitten-Whittingham (BWW) medium on the in vitro development of mouse preimplantation embryos was evaluated. Thioredoxin, superoxide dismutase (SOD), and apotransferrin showed prominent growth-promoting activity, whereas bovine serum albumin (BSA), fatty acid-free BSA, and catalase showed moderate promoting effects. beta-lipoprotein, ovalbumin and hemoglobin were ineffective, and holo-type transferrin and ceruloplasmin were actually toxic to the embryos. These results suggest that the growth-promotive effect of proteins on mouse pronuclear stage embryos is due to their antioxidative action, or to the removal of some free metal ion(s) such as Fe(3+). The mild growth promoting effect of both BSA and fatty acid free BSA suggest that the effect mediated by BSA is not dependent on bound fatty acids, but more likely is due to their antioxidative effect or chelating effect.

Role of protein supplements in the culture of mouse embryos. Publishing Authors By Initials

S Natsuyama,Y Noda,K Narimoto,T Mori,

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Role of protein supplements in the culture of mouse embryos. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 40

Page Numbers: 149-57

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Jul

YEAR: 1993

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LANGUAGE: eng

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AFFILIATION: Department of Gynaecology and Obstetrics, Faculty of Medicine, Kyoto University, Kyoto 606, Japan.

Country: United States

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MEDLINETA: Theriogenology

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