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Metabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation.

Metabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation. Research Abstract Details 

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  • Metabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation. Abstract Text:

    scott t laughlinScott T Laughlin,carolyn r bertozziCarolyn R Bertozzi,scott t laughlinScott T Laughlin,carolyn r bertozziCarolyn R Bertozzi,scott t laughlinScott T Laughlin,carolyn r bertozziCarolyn R Bertozzi,

    Metabolic labeling of glycans with a bioorthogonal chemical reporter such as the azide enables their visualization in cells and organisms as well as the enrichment of specific glycoprotein types for proteomic analysis. This process involves two steps. Azido sugars are fed to cells or organisms and integrated by the glycan biosynthetic machinery into various glycoconjugates. The azido sugars are then covalently tagged with imaging probes or epitope tags, either ex vivo or in vivo, using an azide-specific reaction. This protocol details the syntheses of the azido sugars N-azidoacetylmannosamine (ManNAz), N-azidoacetylgalactosamine (GalNAz), N-azidoacetylglucosamine (GlcNAz) and 6-azidofucose (6AzFuc), and the detection reagents phosphine-FLAG and phosphine-FLAG-His6. Applications to the visualization of cellular glycans and enrichment of glycoproteins for proteomic analysis are described. The synthesis of the azido sugars (ManNAz, GalNAz, GlcNAz or 6AzFuc) or detection reagents (phosphine-FLAG or phosphine-FLAG-His6) can be completed in approximately 1 week. A cell metabolic labeling experiment can be completed in approximately 4 d.

    Metabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation. Publishing Authors By Initials

    st laughlinST Laughlin,cr bertozziCR Bertozzi,st laughlinST Laughlin,cr bertozziCR Bertozzi,st laughlinST Laughlin,cr bertozziCR Bertozzi,

    For similar abstracts research abstracts see: abstracts research

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    Metabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Nature protocols

    VOLUME: 2

    Page Numbers: 2930-44

    Journal Abbreviation:

    ISSN: 1750-2799

    DAY: 16

    MONTH: 11

    YEAR: 2007

    Metabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation. Information

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    LANGUAGE: eng

    NlmUniqueID: 101284307

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    Grant and Affiliation Information for Metabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation.

    AFFILIATION: Department of Chemistry, University of California, Berkeley, California 94720, USA.

    Country: England

    England Research Publication

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    MEDLINETA: Nat Protoc

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