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Isolation and characterization of dendritic cells from common marmosets for preclinical cell therapy studies.

Isolation and characterization of dendritic cells from common marmosets for preclinical cell therapy studies. Research Abstract Details 

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  • Isolation and characterization of dendritic cells from common marmosets for preclinical cell therapy studies. Abstract Text:

    shigeki ohtaShigeki Ohta,yoko uedaYoko Ueda,masae yaguchiMasae Yaguchi,yumi matsuzakiYumi Matsuzaki,masaya nakamuraMasaya Nakamura,yoshiaki toyamaYoshiaki Toyama,yoshikuni taniokaYoshikuni Tanioka,norikazu tamaokiNorikazu Tamaoki,tatsuji nomuraTatsuji Nomura,hideyuki okanoHideyuki Okano,yutaka kawakamiYutaka Kawakami,masahiro todaMasahiro Toda,shigeki ohtaShigeki Ohta,yoko uedaYoko Ueda,masae yaguchiMasae Yaguchi,yumi matsuzakiYumi Matsuzaki,masaya nakamuraMasaya Nakamura,yoshiaki toyamaYoshiaki Toyama,yoshikuni taniokaYoshikuni Tanioka,norikazu tamaokiNorikazu Tamaoki,tatsuji nomuraTatsuji Nomura,hideyuki okanoHideyuki Okano,yutaka kawakamiYutaka Kawakami,masahiro todaMasahiro Toda,

    Dendritic cells (DCs) have important functions as modulators of immune responses, and their ability to activate T cells is of great value in cancer immunotherapy. The isolation of DCs from the peripheral blood of rhesus and African green monkeys has been reported, but the immune system in the common marmoset remains poorly characterized, although it offers many potential advantages for preclinical studies. In the present study, we devised methods, based on techniques developed for mouse and human DC preparation, for isolating DCs from three major tissue sources in the common marmoset: bone marrow (BM), spleen and peripheral blood. Each set of separated cells was analysed using the cell surface DC-associated markers CD11c, CD80, CD83, CD86 and human leucocyte antigen (HLA)-DR, all of which are antibodies against human antigens, and the cells were further characterized both functionally and morphologically as antigen-presenting cells. BM proved to be an excellent cell source for the isolation of DCs intended for preclinical studies on cell therapy, for which large quantities of cells are required. In the BM-derived CD11c(+) cell population, cells exhibiting the characteristic features of DCs were enriched, with the typical DC morphology and the abilities to undergo endocytosis, to secrete interleukin (IL)-12, and to stimulate Xenogenic T cells. Moreover, BM-derived DCs produced the neurotrophic factor NT-3, which is also found in murine splenic DCs. These results suggest that BM-derived DCs from the common marmoset may be useful for biological analysis and for preclinical studies on cell therapy for central nervous system diseases and cancer.

    Isolation and characterization of dendritic cells from common marmosets for preclinical cell therapy studies. Publishing Authors By Initials

    s ohtaS Ohta,y uedaY Ueda,m yaguchiM Yaguchi,y matsuzakiY Matsuzaki,m nakamuraM Nakamura,y toyamaY Toyama,y taniokaY Tanioka,n tamaokiN Tamaoki,t nomuraT Nomura,h okanoH Okano,y kawakamiY Kawakami,m todaM Toda,s ohtaS Ohta,y uedaY Ueda,m yaguchiM Yaguchi,y matsuzakiY Matsuzaki,m nakamuraM Nakamura,y toyamaY Toyama,y taniokaY Tanioka,n tamaokiN Tamaoki,t nomuraT Nomura,h okanoH Okano,y kawakamiY Kawakami,m todaM Toda,

    For similar abstracts research abstracts see: abstracts research

    PUBMED ID PMID:

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    Isolation and characterization of dendritic cells from common marmosets for preclinical cell therapy studies. Journal Published:

    PUBLICATION TYPE: Research Support, Non-U.S. Gov

    Journal: Immunology

    VOLUME: 123

    Page Numbers: 566-74

    Journal Abbreviation: Immunology

    ISSN: 1365-2567

    DAY: 14

    MONTH: 11

    YEAR: 2007

    Isolation and characterization of dendritic cells from common marmosets for preclinical cell therapy studies. Information

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    LANGUAGE: eng

    NlmUniqueID: 374672

    Isolation and characterization of dendritic cells from common marmosets for preclinical cell therapy studies. Keywords Mesh Terms:

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    Grant and Affiliation Information for Isolation and characterization of dendritic cells from common marmosets for preclinical cell therapy studies.

    AFFILIATION: Neuroimmunology Research Group, Keio University School of Medicine, Tokyo, Japan.

    Country: England

    England Research Publication

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    MEDLINETA: Immunology

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