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In vivo imaging of membrane-associated glycans in developing zebrafish.

In vivo imaging of membrane-associated glycans in developing zebrafish. Research Abstract Details 

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  • In vivo imaging of membrane-associated glycans in developing zebrafish. Abstract Text:

    Glycans are attractive targets for molecular imaging but have been inaccessible because of their incompatibility with genetically encoded reporters. We demonstrated the noninvasive imaging of glycans in live developing zebrafish, using a chemical reporter strategy. Zebrafish embryos were treated with an unnatural sugar to metabolically label their cell-surface glycans with azides. Subsequently, the embryos were reacted with fluorophore conjugates by means of copper-free click chemistry, enabling the visualization of glycans in vivo at subcellular resolution during development. At 60 hours after fertilization, we observed an increase in de novo glycan biosynthesis in the jaw region, pectoral fins, and olfactory organs. Using a multicolor detection strategy, we performed a spatiotemporal analysis of glycan expression and trafficking and identified patterns that would be undetectable with conventional molecular imaging approaches.

    In vivo imaging of membrane-associated glycans in developing zebrafish. Publishing Authors By Initials

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    PUBMED ID PMID:

    MEDLINE DATE:

    In vivo imaging of membrane-associated glycans in developing zebrafish. Journal Published:

    PUBLICATION TYPE: Research Support, U.S. Gov't,

    Journal: Science (New York, N.Y.)

    VOLUME: 320

    Page Numbers: 664-7

    Journal Abbreviation: Science

    ISSN: 1095-9203

    DAY: 2

    MONTH: May

    YEAR: 2008

    In vivo imaging of membrane-associated glycans in developing zebrafish. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 404511

    In vivo imaging of membrane-associated glycans in developing zebrafish. Keywords Mesh Terms:

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    Chemical & Substance for Abstract: In vivo imaging of membrane-associated glycans in developing zebrafish. Information

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    Grant and Affiliation Information for In vivo imaging of membrane-associated glycans in developing zebrafish.

    AFFILIATION: Department of Chemistry, University of California, Berkeley, CA 94720, USA.

    Country: United States

    United States Research PublicationUnited States Research Publication

    AGENCY: United States NIGMS

    GRANT: GM061952

    ACRONYM: GM

    MEDLINETA: Science

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