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Domain organization and quaternary structure of the Saccharomyces cerevisiae silent information regulator 3 protein, Sir3p.

Domain organization and quaternary structure of the Saccharomyces cerevisiae silent information regulator 3 protein, Sir3p. Research Abstract Details 

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  • Domain organization and quaternary structure of the Saccharomyces cerevisiae silent information regulator 3 protein, Sir3p. Abstract Text:

    steven j mcbryantSteven J McBryant,christine krauseChristine Krause,jeffrey c hansenJeffrey C Hansen,steven j mcbryantSteven J McBryant,christine krauseChristine Krause,jeffrey c hansenJeffrey C Hansen,

    The silent information regulator protein 3 (Sir3p) functions in the initiation, propagation, and maintenance of transcriptionally silenced chromatin in Saccharomyces cerevisiae. To better understand the physicochemical basis for its effects on chromatin architecture, recombinant full-length S. cerevisiae Sir3p has been purified to near homogeneity on the large-scale and characterized by circular dichroism, limited protease digestion, and analytical ultracentrifugation. Results indicate that the Sir3p monomer has a unique tripartite domain organization, including a nearly 300-amino-acid residue stretch of intrinsically disordered residues that lies internal to its structured N- and C-terminal regions. Sir3p self-associates extensively in moderate salt and at micromolar protein concentrations producing a broad range of oligomers that sediment from 8 to in excess of 85 S. These results provide new insight into Sir3p domain organization and quaternary structure and support a nucleosome bridging model for Sir3p-dependent regulation of chromatin architecture.

    Domain organization and quaternary structure of the Saccharomyces cerevisiae silent information regulator 3 protein, Sir3p. Publishing Authors By Initials

    sj mcbryantSJ McBryant,c krauseC Krause,jc hansenJC Hansen,sj mcbryantSJ McBryant,c krauseC Krause,jc hansenJC Hansen,

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    Domain organization and quaternary structure of the Saccharomyces cerevisiae silent information regulator 3 protein, Sir3p. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Biochemistry

    VOLUME: 45

    Page Numbers: 15941-8

    Journal Abbreviation: Biochemistry

    ISSN: 1520-4995

    DAY: 26

    MONTH: Dec

    YEAR: 2006

    Domain organization and quaternary structure of the Saccharomyces cerevisiae silent information regulator 3 protein, Sir3p. Information

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    LANGUAGE: eng

    NlmUniqueID: 370623

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    Grant and Affiliation Information for Domain organization and quaternary structure of the Saccharomyces cerevisiae silent information regulator 3 protein, Sir3p.

    AFFILIATION: Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523-1870, USA.

    Country: United States

    United States Research PublicationUnited States Research Publication

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    MEDLINETA: Biochemistry

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