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Comparison of photophysical and colloidal properties of biocompatible semiconductor nanocrystals using fluorescence correlation spectroscopy.

Comparison of photophysical and colloidal properties of biocompatible semiconductor nanocrystals using fluorescence correlation spectroscopy. Research Abstract Details 

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  • Comparison of photophysical and colloidal properties of biocompatible semiconductor nanocrystals using fluorescence correlation spectroscopy. Abstract Text:

     doose Doose,james m tsayJames M Tsay,fabien pinaudFabien Pinaud,shimon weissShimon Weiss,

    A number of different surface chemistries have been developed in recent years to render semiconductor nanocrystals (NCs) stable in water and biocompatible. However, most of these surface modifications affect NCs' photophysical properties, calling for a method to simultaneously monitor colloidal and fluorescence properties. Fluorescence correlation spectroscopy (FCS) combined with ensemble spectroscopic methods and Monte Carlo simulations were used to interpret and derive photophysical as well as colloidal properties of four different NC surface treatments. Using a novel FCS scheme with alternating laser excitation at two different intensities, we first ruled out influences from optical gradient forces (optical trapping). We then compared concentration of emitting particles, brightness per particle, saturation intensity, blinking (intermittency), hydrodynamic radius, and propensity for aggregation of the different bioconjugated NCs. This approach was successfully applied during the development and optimization of peptide-coated NCs.

    Comparison of photophysical and colloidal properties of biocompatible semiconductor nanocrystals using fluorescence correlation spectroscopy. Publishing Authors By Initials

    s dooseS Doose,jm tsayJM Tsay,f pinaudF Pinaud,s weissS Weiss,

    For similar investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research abstracts see: investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research

    PUBMED ID PMID:

    MEDLINE DATE:

    Comparison of photophysical and colloidal properties of biocompatible semiconductor nanocrystals using fluorescence correlation spectroscopy. Journal Published:

    PUBLICATION TYPE: Research Support, Non-U.S. Gov

    Journal: Analytical chemistry

    VOLUME: 77

    Page Numbers: 2235-42

    Journal Abbreviation: Anal. Chem.

    ISSN: 0003-2700

    DAY: 1

    MONTH: Apr

    YEAR: 2005

    Comparison of photophysical and colloidal properties of biocompatible semiconductor nanocrystals using fluorescence correlation spectroscopy. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 370536

    Comparison of photophysical and colloidal properties of biocompatible semiconductor nanocrystals using fluorescence correlation spectroscopy. Keywords Mesh Terms:

    KEYWORDS: Spectrometry, Fluorescence

    MESH TERMS: methods

    Chemical & Substance for Abstract: Comparison of photophysical and colloidal properties of biocompatible semiconductor nanocrystals using fluorescence correlation spectroscopy. Information

    Substance Name: Colloids

    Registry Number: 0

    Grant and Affiliation Information for Comparison of photophysical and colloidal properties of biocompatible semiconductor nanocrystals using fluorescence correlation spectroscopy.

    AFFILIATION: Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, California 90095, USA. sdoose@chem.ucla.edu

    Country: United States

    United States Research PublicationUnited States Research Publication

    AGENCY: United States NIBIB

    GRANT: R01 EB000312-05

    ACRONYM: EB

    MEDLINETA: Anal Chem

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